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Literature summary for 4.1.1.19 extracted from

  • Ramakrishna, S.; Adiga, P.R.
    Arginine decarboxylase from Lathyrus sativus seedlings. Purification and properties (1975), Eur. J. Biochem., 59, 377-386.
    View publication on PubMed
Search for more literature for 4.1.1.19

General Stability

General Stability Organism
dialysis against Cys causes irreversible inactivation Lathyrus sativus
freeze-thawing or lyophilization inactivates Lathyrus sativus

Inhibitors

Inhibitors Comment Organism Structure
4-Bromo-3-hydroxybenzoyloxyamine dihydrogen phosphate
-
 Lathyrus sativus
agmatine
-
 Lathyrus sativus
Arcain
-
 Lathyrus sativus
Ca2+
-
 Lathyrus sativus
canavanine
-
 Lathyrus sativus
citrulline
-
 Lathyrus sativus
Co2+
-
 Lathyrus sativus
Cu2+
-
 Lathyrus sativus
D-Arg
-
 Lathyrus sativus
Hg2+
-
 Lathyrus sativus
iodoacetamide
-
 Lathyrus sativus
Mn2+ 0.01-0.1 mM, enhances activity. Inhibition at higher concentrations Lathyrus sativus
N-Carbamoylputrescine
-
 Lathyrus sativus
NaBH4
-
 Lathyrus sativus
NEM
-
 Lathyrus sativus
Orn L-Orn Lathyrus sativus
p-hydroxymercuribenzoate
-
 Lathyrus sativus
phenylhydrazine
-
 Lathyrus sativus
putrescine
-
 Lathyrus sativus
Semicarbazide
-
 Lathyrus sativus
spermidine
-
 Lathyrus sativus
spermine
-
 Lathyrus sativus
Zn2+
-
 Lathyrus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.73
-
 L-Arg
-
 Lathyrus sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Lathyrus sativus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.1 mM increases activity by 35%. 0.01-0.1 mM, enhances activity. Inhibition at higher concentrations Lathyrus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
 gel filtration Lathyrus sativus

Organism

Organism UniProt Comment Textmining
Lathyrus sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lathyrus sativus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Lathyrus sativus
-
seedling
-
 Lathyrus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.495
-
-
 Lathyrus sativus

Storage Stability

Storage Stability Organism
-20°C, labile, even in presence of 20% glycerol Lathyrus sativus
0-2°C, 70% loss of activity after 5 days Lathyrus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine
-
 Lathyrus sativus agmatine + CO2
-
 ?
L-Asp 2.6% of the activity with L-Arg Lathyrus sativus ?
-
 ?
L-Glu 2.1% of the activity with L-Arg Lathyrus sativus ?
-
 ?
L-Phe 1.4% of the activity with L-Arg Lathyrus sativus phenylethylamine + CO2
-
 ?
L-Pro 3.8% of the activity with L-Arg Lathyrus sativus ?
-
 ?
L-Ser 4.4% of the activity with L-Arg Lathyrus sativus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 36000-36500, SDS-PAGE Lathyrus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Lathyrus sativus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 45 37°C: about 50% of maximal activity, 45°C: maximal activity Lathyrus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Lathyrus sativus