Cloned (Comment) | Organism |
---|---|
gene ldc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the soluble His-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative real-time reverse transcription PCR enzyme expression analysis | Morus alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0192 | - |
L-lysine | recombinant His-tagged enzyme, pH 8.0, 37°C | Morus alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Morus alba | - |
cadaverine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morus alba | A0A2Z4EVE5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Morus alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Morus alba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Morus alba | cadaverine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, recombinant His-tagged enzyme, SDS-PAGE | Morus alba |
Synonyms | Comment | Organism |
---|---|---|
LDC | - |
Morus alba |
MaLDC | - |
Morus alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Morus alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Morus alba |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Morus alba |
General Information | Comment | Organism |
---|---|---|
physiological function | lysine decarboxylase MaLDC is involved in the biosynthesis of DNJ alkaloids. 1-Deoxynojirimycin (DNJ) is the main bioactive compound of Morus alba and has pharmacological effects in humans, including blood sugar level regulation and antiviral activity. The enzyme expression is correlated with DNJ content in leaves | Morus alba |