Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.18 extracted from

  • Kou, F.; Zhao, J.; Liu, J.; Shen, J.; Ye, Q.; Zheng, P.; Li, Z.; Sun, J.; Ma, Y.
    Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH (2016), J. Mol. Catal. B, 133, S88-S94 .
No PubMed abstract available
Search for more literature for 4.1.1.18

Application

Application Comment Organism
synthesis enzyme AsLdc has a high potential for the industrial production of cadaverine Aliivibrio salmonicida

Cloned(Commentary)

Cloned (Comment) Organism
gene cadA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Aliivibrio salmonicida

Protein Variants

Protein Variants Comment Organism
additional information when used for whole-cell biotransformation of L-lysine to cadaverine at pH 7.5, 37°C, recombinant AsLdc in Escherichia coli cells completes the transformation within 7 h, method optimiztaion and comprisons, overview Aliivibrio salmonicida

Inhibitors

Inhibitors Comment Organism Structure
cadaverine competitive inhibition mechanism, 90% inhibition at 10 mM Aliivibrio salmonicida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.92
-
 L-lysine recombinant enzyme, pH 7.5, 37°C Aliivibrio salmonicida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
158000
-
 dimeric enzyme, native PAGE Aliivibrio salmonicida
780000
-
 decameric enzyme, native PAGE Aliivibrio salmonicida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine Aliivibrio salmonicida
-
 cadaverine + CO2
-
 ?
additional information Aliivibrio salmonicida when used for whole-cell biotransformation of L-lysine to cadaverine at pH 7.5, 37°C, recombinant AsLdc in Escherichia coli cells completes the transformation within 7 h ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aliivibrio salmonicida B6EKJ5 i.e. Vibrio salmonicida
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Aliivibrio salmonicida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
94.1
-
 purified recombinant enzyme, pH 8.5, 37°C, substrate L-lysine Aliivibrio salmonicida
205.1
-
 purified recombinant enzyme, pH 7.5, 37°C, substrate L-lysine Aliivibrio salmonicida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
 Aliivibrio salmonicida cadaverine + CO2
-
 ?
L-lysine 100% conversion by the recombinant enzyme at pH 7.5 Aliivibrio salmonicida cadaverine + CO2
-
 ?
additional information when used for whole-cell biotransformation of L-lysine to cadaverine at pH 7.5, 37°C, recombinant AsLdc in Escherichia coli cells completes the transformation within 7 h Aliivibrio salmonicida ?
-
 ?

Subunits

Subunits Comment Organism
decamer
-
 Aliivibrio salmonicida
dimer
-
 Aliivibrio salmonicida
More a mixture of dimers (about 158 kDa) and decamers (about 780 kDa) is present for the enzyme at pH 7.5. Decamers are dominant for AsLdc, dependent on the pH: the highest proportion of decamer for AsLdc (78%) is observed at pH 7.5, while it is reduced to 30% and 57% at pH 5.0 and pH 8.5, respectively Aliivibrio salmonicida

Synonyms

Synonyms Comment Organism
AsLdc
-
 Aliivibrio salmonicida
CadA
-
 Aliivibrio salmonicida
LDC
-
 Aliivibrio salmonicida
VSAL_I2491
-
 Aliivibrio salmonicida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aliivibrio salmonicida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 purified recombinant enzyme AsLdc, 12 h, pH 7.5, residual activity is 96.3% Aliivibrio salmonicida
50
-
 purified recombinant enzyme AsLdc, 12 h, pH 7.5, residual activity is 75.2% Aliivibrio salmonicida
79
-
 Tm of recombinant enzyme AsLdc Aliivibrio salmonicida
80
-
 purified recombinant enzyme AsLdc, 12 h, pH 7.5, residual activity is about 50% Aliivibrio salmonicida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Aliivibrio salmonicida

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 high activity within this range Aliivibrio salmonicida

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
 purified recombinant enzyme AsLdc, 10 h, 37°C, residual activity is 71% Aliivibrio salmonicida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Aliivibrio salmonicida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.46
-
 cadaverine recombinant enzyme, pH 7.5, 37°C Aliivibrio salmonicida