Cloned (Comment) | Organism |
---|---|
gene ldc, recombinant expression of codon-optimized enzyme in Escherichia coli K-12 resulting in strain LN20, optimizing the sequence located in the region of the ribosome-binding site in order to enhance translation efficiency further improves the system, subcloning in Escherichia coli TOP10 cells. Batch and fed-batch production of Klebsiella oxytoca lysine decarboxylase from several modifed strains, overview | Klebsiella oxytoca |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of mutant Ldc-co with increased lysine decarboxylase ability. Codon optimization of the gene encoding the enzyme is carried on for the heterologous expression in Escherichia coli. Identification of mutant lysine decarboxylase enzymes with enhanced cadaverine-production ability. Together, these modifications increase cadaverine production in the system by 50%, and the system has a yield of 80% from lysine-HCl, the system to produce cadaverine using the lysine decarboxylase from Klebsiella oxytoca performs at a level that is competitive with the traditional systems using the Escherichia coli lysine decarboxylases in both lab-scale and batch fermentation conditions. Generation of several mutant strains and evaluation, overview | Klebsiella oxytoca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Klebsiella oxytoca | - |
cadaverine + CO2 | - |
? | |
L-lysine | Klebsiella oxytoca DSM 6673 | - |
cadaverine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | - |
- |
- |
Klebsiella oxytoca DSM 6673 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Klebsiella oxytoca | cadaverine + CO2 | - |
? | |
L-lysine | - |
Klebsiella oxytoca DSM 6673 | cadaverine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LDC | - |
Klebsiella oxytoca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Klebsiella oxytoca |