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Literature summary for 4.1.1.18 extracted from
- Novel characteristics of Selenomonas ruminantium lysine decarboxylase capable of decarboxylating both L-lysine and L-ornithine (1999), Biosci. Biotechnol. Biochem., 63, 1063-1069.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-alpha-Difluoromethyllysine | competitive | Selenomonas ruminantium |  |
| DL-alpha-difluoromethylornithine | competitive and irreversible | Selenomonas ruminantium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.63 | - |
L-Lys | pH 6.0, 40°C | Selenomonas ruminantium | |
| 1.2 | - |
L-Orn | pH 6.0, 40°C | Selenomonas ruminantium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Selenomonas ruminantium |
| 88000 | - |
gel filtration | Selenomonas ruminantium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Lys | Selenomonas ruminantium | constitutive enzyme is involved in synthesis of cadaverine, which is an essential constituent of the peptidoglycan for normal cell growth | Cadaverine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Selenomonas ruminantium | O50657 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Selenomonas ruminantium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | drastic descent of enzyme activity owing to degradation of LDC at entry into the stationary phase of cell growth | Selenomonas ruminantium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.88 | - |
- |
Selenomonas ruminantium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for one month in presence of 20% glycerol, loss of activity within 2 weeks without glycerol | Selenomonas ruminantium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Lys | - |
Selenomonas ruminantium | Cadaverine + CO2 | - |
? | |
| L-Lys | constitutive enzyme is involved in synthesis of cadaverine, which is an essential constituent of the peptidoglycan for normal cell growth | Selenomonas ruminantium | Cadaverine + CO2 | - |
? | |
| L-Orn | - |
Selenomonas ruminantium | Putrescine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Selenomonas ruminantium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LDC | - |
Selenomonas ruminantium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Selenomonas ruminantium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
30 min, 50% loss of activity without pyridoxal-5'-phosphate | Selenomonas ruminantium |
| 50 | - |
30 min, 50% loss of activity in presence of pyridoxal 5'-phosphate | Selenomonas ruminantium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 289.8 | - |
L-Orn | pH 6.0, 40°C | Selenomonas ruminantium | |
| 636 | - |
L-Lys | pH 6.0, 40°C | Selenomonas ruminantium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Selenomonas ruminantium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | stable | Selenomonas ruminantium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Selenomonas ruminantium | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00525 | - |
DL-alpha-difluoromethylornithine | pH 6.0, 40°C, reaction with L-Lys | Selenomonas ruminantium | |
| 0.0082 | - |
DL-alpha-difluoromethylornithine | pH 6.0, 40°C, reaction with L-Orn | Selenomonas ruminantium | |
| 0.0175 | - |
DL-alpha-Difluoromethyllysine | pH 6.0, 40°C, reaction with L-Lys | Selenomonas ruminantium | |
| 0.028 | - |
DL-alpha-Difluoromethyllysine | pH 6.0, 40°C, reaction with L-Orn | Selenomonas ruminantium | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Selenomonas ruminantium | - |
- |
4.9 |
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