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Literature summary for 4.1.1.17 extracted from
- Effect of ornithine decarboxylase and norspermidine in modulating cell division in the green alga Chlamydomonas reinhardtii (2018), Plant Physiol. Biochem., 123, 125-131 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ODC1 | Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-difluoromethylornithine | DFMO, a suicide inhibitor, at 5 mM for 72 h, it inhibits membrane-associated ODC activity. Following exposure to alpha-DFMO, the putrescine content in the cells declineds, while the norspermidine content increases over 2fold. Partial inhibition of putrescine synthesis by alpha-DFMO results in increased norspermidine production in the cells suggesting that the suicide inhibitor may also act as an abiotic stress agent | Chlamydomonas reinhardtii |  |
| additional information | alpha-difluoromethylarginine, DFMA, a suicide inhibitor, does not inhibit the enzyme | Chlamydomonas reinhardtii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme is found in the soluble and the pellet fractions | Chlamydomonas reinhardtii | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine | Chlamydomonas reinhardtii | - |
putrescine + CO2 | - |
? | |
| L-ornithine | Chlamydomonas reinhardtii CC-125 | - |
putrescine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | A8J7E8 | and CC-406 cell-wall mutant | - |
| Chlamydomonas reinhardtii CC-125 | A8J7E8 | and CC-406 cell-wall mutant | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine | - |
Chlamydomonas reinhardtii | putrescine + CO2 | - |
? | |
| L-ornithine | - |
Chlamydomonas reinhardtii CC-125 | putrescine + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ODC | - |
Chlamydomonas reinhardtii |
| ODC1 | - |
Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Chlamydomonas reinhardtii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | ODC is the major route to polyamine formation in the Chlamydomonas CC-406 cell-wall mutant, in contrast to the preferential ADC route reported for Chlorella vulgaris, suggesting that significant species differences exist in biosynthetic pathways which modulate endogenous polyamine levels in green algae | Chlamydomonas reinhardtii |
| metabolism | putrescine is the major polyamine in both free (88%) and membrane-bound fractions (93%) in Chlamydomonas reinhardtii, while norspermidine is the next most abundant in these fractions accounting for 11% and 6%, respectively. Low levels of diaminopropane, spermidine and spermine are also observed although no cadaverine or norspermine are detected. Ornithine decarboxylase (ODC) activity is almost five times higher than arginine decarboxylase (ADC, EC 4.1.1.19) and is the major route of putrescine synthesis. Following exposure to ODC inhibitor alpha-DFMO, the putrescine content in the cells declines, while the norspermidine content increases over 2fold. Addition of norspermidine to cultures stimulates cell division mimicking the effects observed using alpha-DFMO and also reverses the inhibitory effects of cyclohexylamine on growth | Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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