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Literature summary for 4.1.1.17 extracted from

  • Porat, Z.; Landau, G.; Bercovich, Z.; Krutauz, D.; Glickman, M.; Kahana, C.
    Yeast antizyme mediates degradation of yeast ornithine decarboxylase by yeast but not by mammalian proteasome - new insights on yeast antizyme (2008), J. Biol. Chem., 283, 4528-4534 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ODC, HEK-293 cells are transiently transfected with yeast ODC, mammalian ODC or the chimerical proteins together with either yeast Az or mammalian Az. Overexpression of yAz in yeast cells results in polyamine depletion and growth inhibition Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information the stability of yODC in mammalian cells is not a result of the absence of a compatible antizyme Az or lack of a C-terminal-destabilizing signal found on the mammalian enzyme, but is rather a result of the inability of the mammalian proteasome to degrade yeast ODC Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine Saccharomyces cerevisiae
-
putrescine + CO2
-
?
L-ornithine Saccharomyces cerevisiae BY4741
-
putrescine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P08432 from strain ATCC 204508 / S288c
-
Saccharomyces cerevisiae BY4741 P08432 from strain ATCC 204508 / S288c
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the stability of yODC in mammalian cells is not a result of the absence of a compatible antizyme Az or lack of a C-terminal-destabilizing signal found on the mammalian enzyme, but is rather a result of the inability of the mammalian proteasome to degrade yeast ODC. Yeast antizyme (yAz) stimulates the degradation of yeast ODC by the yeast proteasome, interaction with yAz provokes degradation of yODC by yeast but not by mammalian proteasomes Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine
-
Saccharomyces cerevisiae putrescine + CO2
-
?
L-ornithine
-
Saccharomyces cerevisiae BY4741 putrescine + CO2
-
?

Synonyms

Synonyms Comment Organism
ODC
-
Saccharomyces cerevisiae
YODC
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism ornithine decarboxylase (ODC) is the first and rate-limiting enzyme in the biosynthesis pathway of polyamines. ODC decarboxylates ornithine to form putrescine, which is further converted to spermidine and spermine via the action of spermidine and spermine synthase, respectively. Mammalian antizyme (mAz) is a central element of a feedback circuit regulating cellular polyamines by accelerating ODC degradation and inhibiting polyamine uptake Saccharomyces cerevisiae
additional information the stability of yODC in mammalian cells is not a result of the absence of a compatible Aantizyme Az or lack of a C-terminal-destabilizing signal found on the mammalian enzyme, but is rather a result of the inability of the mammalian proteasome to degrade yeast ODC. Overexpression of yAz in yeast cells results in polyamine depletion and growth inhibition mainly through inhibiting enzyme ODC Saccharomyces cerevisiae
physiological function ornithine decarboxylase (ODC) is the first and rate-limiting enzyme in the biosynthesis pathway of polyamines. ODC decarboxylates ornithine to form putrescine Saccharomyces cerevisiae