Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.17 extracted from

  • Preeti, R.J.; Tapas, S.; Kumar, P.; Madhubala, R.; Tomar, S.
    Biochemical, mutational and in silico structural evidence for a functional dimeric form of the ornithine decarboxylase from Entamoeba histolytica (2012), PLoS Negl. Trop. Dis., 6, e1559.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Entamoeba histolytica

Crystallization (Commentary)

Crystallization (Comment) Organism
circular dichroism analysis reveals 39% alpha-helix, 25% beta-sheets and 36% random coils. Modeling of the enzyme dimer shows two separate active sites at the dimer interface with Lys57 and Cys334 residues of opposite monomers contributing to each active site Entamoeba histolytica

Protein Variants

Protein Variants Comment Organism
C334A mutation of a putative active site at the dimer interface, completely abolishes enzyme activity. Partial restoration of the enzyme activity is observed when inactive K57A and C334A mutants are mixed, confirming that the dimer is the active form Entamoeba histolytica
G361Y mutation at dimer interface, abolishes enzyme activity and destabilizes the dimer Entamoeba histolytica
K157A mutation at dimer interface, abolishes enzyme activity and destabilizes the dimer Entamoeba histolytica
K57A mutation of a putative active site at the dimer interface, completely abolishes enzyme activity. Partial restoration of the enzyme activity is observed when inactive K57A and C334A mutants are mixed, confirming that the dimer is the active form Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44558
-
2 * 44558, MALDI-TOF Entamoeba histolytica
46000
-
2 * 46000, SDS-PAGE Entamoeba histolytica
90000
-
gel filtration and SDS-PAGE, protein cross-linked by glutaraldehyde Entamoeba histolytica
90670
-
dimeric enzyme, MALDI-TOF Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q58P26
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine
-
Entamoeba histolytica putrescine + CO2
-
?
additional information no activity with D-ornithine Entamoeba histolytica ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Entamoeba histolytica
dimer 2 * 44558, MALDI-TOF Entamoeba histolytica