Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Entamoeba histolytica |
Crystallization (Comment) | Organism |
---|---|
circular dichroism analysis reveals 39% alpha-helix, 25% beta-sheets and 36% random coils. Modeling of the enzyme dimer shows two separate active sites at the dimer interface with Lys57 and Cys334 residues of opposite monomers contributing to each active site | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
C334A | mutation of a putative active site at the dimer interface, completely abolishes enzyme activity. Partial restoration of the enzyme activity is observed when inactive K57A and C334A mutants are mixed, confirming that the dimer is the active form | Entamoeba histolytica |
G361Y | mutation at dimer interface, abolishes enzyme activity and destabilizes the dimer | Entamoeba histolytica |
K157A | mutation at dimer interface, abolishes enzyme activity and destabilizes the dimer | Entamoeba histolytica |
K57A | mutation of a putative active site at the dimer interface, completely abolishes enzyme activity. Partial restoration of the enzyme activity is observed when inactive K57A and C334A mutants are mixed, confirming that the dimer is the active form | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44558 | - |
2 * 44558, MALDI-TOF | Entamoeba histolytica |
46000 | - |
2 * 46000, SDS-PAGE | Entamoeba histolytica |
90000 | - |
gel filtration and SDS-PAGE, protein cross-linked by glutaraldehyde | Entamoeba histolytica |
90670 | - |
dimeric enzyme, MALDI-TOF | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q58P26 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | - |
Entamoeba histolytica | putrescine + CO2 | - |
? | |
additional information | no activity with D-ornithine | Entamoeba histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, SDS-PAGE | Entamoeba histolytica |
dimer | 2 * 44558, MALDI-TOF | Entamoeba histolytica |