Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ornithine decarboxylase paralogue | ornithine decarboxylase paralogue acts as a regulator of ODC activity and inhibits its proteasomal degradation | Homo sapiens | |
ornithine decarboxylase paralogue | ODCp, degraded by the ubiquitin-dependent pathway. The ornithine decarboxylase paralogue antizyme inhibitor ODCp acts as a regulator of ODC activity and inhibits its proteasomal degradation. ODCp binds antizymes, AZs, with higher affinity than does ODC and releases active ODC from catalytically inactive ODC-AZ complexes, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
for transient transfection experiments, ODC cDNAs are cloned into the p3xFLAG-CMV-10 vector, transient transfections of COS-7 cells, used in the ODC activity assay, are performed. | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antizyme | the ornithine decarboxylase paralogue antizyme inhibitor ODCp acts as a regulator of ODC activity and inhibits its proteasomal degradation. ODCp binds antizymes, AZs, with higher affinity than does ODC and releases active ODC from catalytically inactive ODC-AZ complexes, overview | Homo sapiens | |
antizyme 1 | ODC is regulated by specific inhibitors, antizymes which in turn are inhibited by antizyme inhibitors. ODC alone is not degraded in reticulocyte lysate, whereas in the presence of antizyme 1, the degradation occurs rapidly. When antizyme inhibitor or ODC paralogue is added to the mixture, the antizyme 1-induced degradation of ODC is prevented. | Homo sapiens | |
additional information | ODC is degraded in an ubiquitin-independent manner mediated by antizyme 1 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | Homo sapiens | - |
putrescine + CO2 | - |
? | |
L-ornithine | Homo sapiens | rate-limiting enzyme in polyamine biosynthesis | putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ODC is expressed in all types of cells | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
The ODC activity is determined by measuring the release of CO2 from L-ornithine, in vitro-translated proteins are combined in the proportion 2.5:1:7 (ODC:antizyme 1:ODC paralogue/AZIN2), ODC activity increases in the reaction mixtures containing ODC with either ODC paralogue or antizyme inhibitor together with antizyme 1 compared with the control reaction containing ODC with antizyme 1 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | - |
Homo sapiens | putrescine + CO2 | - |
? | |
L-ornithine | rate-limiting enzyme in polyamine biosynthesis | Homo sapiens | putrescine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Homo sapiens |
ornithine decarboxylase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |