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Literature summary for 4.1.1.17 extracted from

  • Kanerva, K.; Makitie, L.T.; Pelander, A.; Heiskala, M.; Andersson, L.C.
    Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase (2008), Biochem. J., 409, 187-192.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ornithine decarboxylase paralogue ornithine decarboxylase paralogue acts as a regulator of ODC activity and inhibits its proteasomal degradation Homo sapiens
ornithine decarboxylase paralogue ODCp, degraded by the ubiquitin-dependent pathway. The ornithine decarboxylase paralogue antizyme inhibitor ODCp acts as a regulator of ODC activity and inhibits its proteasomal degradation. ODCp binds antizymes, AZs, with higher affinity than does ODC and releases active ODC from catalytically inactive ODC-AZ complexes, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
for transient transfection experiments, ODC cDNAs are cloned into the p3xFLAG-CMV-10 vector, transient transfections of COS-7 cells, used in the ODC activity assay, are performed. Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antizyme the ornithine decarboxylase paralogue antizyme inhibitor ODCp acts as a regulator of ODC activity and inhibits its proteasomal degradation. ODCp binds antizymes, AZs, with higher affinity than does ODC and releases active ODC from catalytically inactive ODC-AZ complexes, overview Homo sapiens
antizyme 1 ODC is regulated by specific inhibitors, antizymes which in turn are inhibited by antizyme inhibitors. ODC alone is not degraded in reticulocyte lysate, whereas in the presence of antizyme 1, the degradation occurs rapidly. When antizyme inhibitor or ODC paralogue is added to the mixture, the antizyme 1-induced degradation of ODC is prevented. Homo sapiens
additional information ODC is degraded in an ubiquitin-independent manner mediated by antizyme 1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine Homo sapiens
-
putrescine + CO2
-
?
L-ornithine Homo sapiens rate-limiting enzyme in polyamine biosynthesis putrescine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information ODC is expressed in all types of cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
The ODC activity is determined by measuring the release of CO2 from L-ornithine, in vitro-translated proteins are combined in the proportion 2.5:1:7 (ODC:antizyme 1:ODC paralogue/AZIN2), ODC activity increases in the reaction mixtures containing ODC with either ODC paralogue or antizyme inhibitor together with antizyme 1 compared with the control reaction containing ODC with antizyme 1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine
-
Homo sapiens putrescine + CO2
-
?
L-ornithine rate-limiting enzyme in polyamine biosynthesis Homo sapiens putrescine + CO2
-
?

Synonyms

Synonyms Comment Organism
ODC
-
Homo sapiens
ornithine decarboxylase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Homo sapiens