Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BLR(DE3) | Leishmania donovani |
expression of His6-tagged enzyme in Escherichia coli strain BLR(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme in complex with inhibitor 1-amino-oxy-3-aminopropane or with both inhibitor and cofactor, sitting drop vapour diffusion method, 15C, 6 mg/ml protein in 25 mM HEPES, pH 7.2, 2 mM DTT, 0.5 mM EDTA, 0.02% Brij 35, 1 mM PMSF and 2 mM 1-amino-oxy-3-aminopropane, with or without 0.02 mM pyridoxal 5'-phosphate, mixed with well solution containing 25% v/v PEG 3350, 0.2 M ammonium acetate and 0.1 M Bis-Tris, pH 6.5, 2 days, needle-cluster crystals, single crystals are obtained by using a well solution containing 18% v/v PEG 3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.5, and 3 mg/ml protein concentration, X-ray diffraction structure determination and analysis at 1.9-3.0 A resolution, molecular replacement | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-amino-oxy-3-aminopropane | i.e. APA, analysis of the mode of the inhibitor binding to the enzyme, no oxime formation between APA and pyridoxal 5'-phosphate, homology modelling, overview | Homo sapiens | |
1-amino-oxy-3-aminopropane | i.e. APA, analysis of the mode of the inhibitor binding to the enzyme, no oxime formation between APA and pyridoxal 5'-phosphate, determined from the human enzyme-inhibitor cyrstal structure analysis and homology modelling, overview | Leishmania donovani | |
alpha-difluoromethylornithine | irreversible inhibitor, a curative agent of West African sleeping sickness | Homo sapiens | |
alpha-difluoromethylornithine | irreversible inhibitor | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | Leishmania donovani | ODC is the first committed enzyme in the polyamine biosynthesis pathway | putrescine + CO2 | - |
? | |
L-ornithine | Homo sapiens | ODC is the first committed enzyme in the polyamine biosynthesis pathway | putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11926 | - |
- |
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine | - |
Leishmania donovani | putrescine + CO2 | - |
? | |
L-ornithine | - |
Homo sapiens | putrescine + CO2 | - |
? | |
L-ornithine | ODC is the first committed enzyme in the polyamine biosynthesis pathway | Leishmania donovani | putrescine + CO2 | - |
? | |
L-ornithine | ODC is the first committed enzyme in the polyamine biosynthesis pathway | Homo sapiens | putrescine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODC | - |
Leishmania donovani |
ODC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania donovani |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leishmania donovani |
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Leishmania donovani | |
pyridoxal 5'-phosphate | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
1-amino-oxy-3-aminopropane | pH 7.5, 37°C, recombinant enzyme | Leishmania donovani | |
0.000001 | - |
1-amino-oxy-3-aminopropane | pH 7.5, 37°C, recombinant enzyme | Homo sapiens |