Literature summary for 4.1.1.17 extracted from

Pantazaki, A.A.; Anagnostopoulos, C.G.; Lioliou, E.E.; Kyriakidis, D.A.
Characterization of ornithine decarboxylase and regulation by its antizyme in Thermus thermophilus (1999), Mol. Cell. Biochem., 195, 55-64.

Search for more literature for 4.1.1.17

Activating Compound

Activating Compound	Comment	Organism	Structure
adenosine	approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 2fold activation of native ODC	Thermus thermophilus
ATP	approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 1.5fold activation of native ODC	Thermus thermophilus
GMP	approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 1.2fold activation of native ODC	Thermus thermophilus
GTP	approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 2.5fold activation of native ODC	Thermus thermophilus
phosphoserine	2 mM, 1.5fold activation	Thermus thermophilus
spermidine	2 mM, 40% activation	Thermus thermophilus
spermine	2 mM, 140% activation	Thermus thermophilus
UTP	approx. 10fold activation of dephosphorylated ODC at 1 mM, approx. 2fold activation of native ODC	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
antizyme	non-competitive inhibitor protein isolated from Thermus thermophilus or Escherichia coli, almost complete inhibition at higer concentrations	Escherichia coli
antizyme	non-competitive inhibitor protein isolated from Thermus thermophilus	Thermus thermophilus
L-arginine	competitive inhibition	Thermus thermophilus
L-lysine	competitive inhibition	Thermus thermophilus
putrescine	slight inhibition	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.5	-	L-ornithine	-	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	2 * 68000, SDS-PAGE	Thermus thermophilus
135000	-	gel filtration	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-ornithine	Escherichia coli	-	putrescine + CO2	-	?
L-ornithine	Thermus thermophilus	involved in polyamine biosynthesis	putrescine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Thermus thermophilus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	inactive after incubation with alkaline phosphatase	Thermus thermophilus

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
ammonium sulfate, DEAE-Biogel A, heparin-Sepharose, Sephadex G 150, Phenyl-Sepharose, Lysine-Sepharose, pyridoxamine-affi gel	Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.078	-	-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ornithine	-	Thermus thermophilus	putrescine + CO2	-	?
L-ornithine	-	Escherichia coli	putrescine + CO2	-	?
L-ornithine	involved in polyamine biosynthesis	Thermus thermophilus	putrescine + CO2	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 68000, SDS-PAGE	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
ODC	-	Thermus thermophilus
ODC	-	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	85	85% loss of activity after 15 min at 75°C, more than 95% loss of activity after 15 min at 85°C and above	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Thermus thermophilus

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Release 2023.1 (January 2023)