Literature summary for 4.1.1.17 extracted from

Jackson, L.K.; Brooks, H.B.; Osterman, A.L.; Goldsmith, E.J.; Phillips, M.A.
Altering the reaction specificity of eukaryotic ornithine decarboxylase (2000), Biochemistry, 39, 11247-11257.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment)	Organism
ODC complexed with putrescine, 25 mg/ml ODC in 10 mM HEPES, pH 7.2, 450 mM NaCl, 10 mM dithiothreitol, 0.5 mM EDTA, 0.01% Brij-20, are preincubated with 15 mM D-ornithine for 10 min at room temperatur before crystallization, crystals are obtained at 16°C by vapor diffusion mixing equal volumes of ODC-D-ornithine mixture and well solution containing 20% polyethylene glycol 3350, 200 mM NaOAc, 100 mM HEPES, pH 7.5 and 10 mM dithiothreitol	Trypanosoma brucei

Crystallization (Comment)

Organism

ODC complexed with putrescine, 25 mg/ml ODC in 10 mM HEPES, pH 7.2, 450 mM NaCl, 10 mM dithiothreitol, 0.5 mM EDTA, 0.01% Brij-20, are preincubated with 15 mM D-ornithine for 10 min at room temperatur before crystallization, crystals are obtained at 16°C by vapor diffusion mixing equal volumes of ODC-D-ornithine mixture and well solution containing 20% polyethylene glycol 3350, 200 mM NaOAc, 100 mM HEPES, pH 7.5 and 10 mM dithiothreitol

Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
C360A	2% of wild-type activity	Trypanosoma brucei
C360S	0.1% of wild-type activity	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha-difluoromethylornithine	-	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.054	-	L-ornithine	C360S mutant	Trypanosoma brucei
0.1	-	L-ornithine	C360A mutant	Trypanosoma brucei
0.4	-	L-ornithine	-	Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-ornithine	Trypanosoma brucei	-	putrescine + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	P07805	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant ODC	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ornithine	-	Trypanosoma brucei	putrescine + CO2	-	?

Synonyms

Synonyms	Comment	Organism
ODC	-	Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
15	-	L-ornithine	-	Trypanosoma brucei

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Trypanosoma brucei