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Literature summary for 4.1.1.15 extracted from

  • Choi, J.W.; Yim, S.S.; Lee, S.H.; Kang, T.J.; Park, S.J.; Jeong, K.J.
    Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range (2015), Microb. Cell Fact., 14, 21 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene gadB, recombinnat enzyme expression in Corynebacterium glutamicum, evaluation of PH36, PI16, and PL26 promoters for optimal expression and different pH value for engineered cultures Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information Corynebacterium glutamicum, the major L-glutamate producing microorganism, has been engineered to achieve direct fermentative production of GABA from glucose, method optimization, overview. In the fed-batch cultivation at pH 5.0, which is not preferable condition for cell growth, much less glutamate might be synthesized, which results in much lower production of GABA compared with that of pH 6.0. In the culture at pH 6.0, increased GAD activity can be balanced for glutamate synthesis to result in high-level production of GABA of up to 38.6 g/l. Corynebacterium glutamicum expressing Escherichia coli GAD mutant under the strong PH36 promoter produces GABA to the concentration of 5.89 g/l in GP1 medium at pH 7.0, which is 17fold higher than that obtained by Corynebacterium glutamicum expressing wild-type Escherichia coli GAD in the same condition (0.34 g/l). Optimized fed-batch culture of Corynebacterium glutamicum expressing Escherichia coli GAD mutant in GP1 medium containing 0.05 mg/l of biotin at pH 6.0, 30°C, results in the highest GABA concentration of 38.6 g/l with the productivity of 0.536 g/l/h Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Escherichia coli
-
 4-aminobutanoate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Escherichia coli 4-aminobutanoate + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 about Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli