Application | Comment | Organism |
---|---|---|
food industry | the recombinant Lactobacillus plantarum strain overexpressing the Lactobacillus plantarum enzyme can be used as a starter culture for direct incorporation into a food system during fermentation for production of GABA-rich products | Lactiplantibacillus plantarum |
Cloned (Comment) | Organism |
---|---|
gene gad, DNA and amino acid sequence determimation and analysis, sequence comparisons, recombinant overexpression in Lactobacillus plantarum strain Taj-Apis362 | Lactiplantibacillus plantarum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the optimal fermentation conditions for GABA production from recombinant Lactobacillus plantarum strain overexpressing the Lactobacillus plantarum enzyme using response surface methodology are a glutamic acid concentration of 497.973 mM, temperature 36°C, pH 5.31, and time 60 h. Under these conditions, maximum GABA concentration obtained is 11.09 mM. GAD activity of the cell extract released by the recombinant strain (167.2 units/ml/min) is sevenfold greater than the GAD activity of wild-type cells (23.5 units/ml/min). Modelling of recombinant enzyme production, overview | Lactiplantibacillus plantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Lactiplantibacillus plantarum | - |
4-aminobutanoate + CO2 | - |
? | |
L-glutamate | Lactiplantibacillus plantarum Taj-Apis362 | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | W0P1N8 | - |
- |
Lactiplantibacillus plantarum Taj-Apis362 | W0P1N8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Lactiplantibacillus plantarum | 4-aminobutanoate + CO2 | - |
? | |
L-glutamate | - |
Lactiplantibacillus plantarum Taj-Apis362 | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, recombinant His-tagged enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Lactiplantibacillus plantarum |
LbGAD | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Lactiplantibacillus plantarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Lactiplantibacillus plantarum |