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Literature summary for 4.1.1.15 extracted from
- Cloning and expression of rice glutamate decarboxylase (GAD) in Escherichia coli (2017), Cell. Mol. Biol. (Noisy-le-grand), 63, 106-108 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| IPTG-induced functional recombinant expression of His-tagged Oryza sativa GAD isozymes in Escherichia coli strain MC1061 at 28°C and 37°C, method optimization | Oryza sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | method evaluation for production of plant enzyme in bacteria at industrial scale | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Oryza sativa | - |
4-aminobutanoate + CO2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Oryza sativa GAD isozymes from Escherichia coli strain MC1061 by nickel affinity chromatography wih varying concentrations of imidazole, best at 100 mM | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Oryza sativa | 4-aminobutanoate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Oryza sativa | |
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Release 2023.1 (January 2023)
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