Cloned (Comment) | Organism |
---|---|
gene gadB, recombinant expression in Corynebacterium glutamicum strain ATCC 13032 with or without gene pknG knockout | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the Escherichia coli glutamate decarboxylase is expressed in Corynebacterium glutamicum for production of gamma-aminobutyric acid (GABA), a building block of the biodegradable plastic polyamide 4. Disruption of gene pknG, encoding serine/threonine protein kinase G (EC 2.7.11.1), enhances recombinant production of gamma-aminobutyric acid through reduction of the 2-oxoglutarate dehydrogenase complex (ODHC) activity regulation, PknG catalyzes the phosphorylation of OdhI, a 15 kDa subunit of ODHC, which reverses the inhibition of the ODHC activity. Strain GAD produces 13.06 g/l of GABA in 120 hours, consuming 83.62 g/l of glucose, strain GADDELTApknG produces 0.272 g/l of GABA in 120 hours, consuming less glucose | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Escherichia coli | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A5YKJ2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Escherichia coli | 4-aminobutanoate + CO2 | - |
? | |
L-glutamate | alpha-decarboxylation | Escherichia coli | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAD B | - |
Escherichia coli |
GadB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |