Application | Comment | Organism |
---|---|---|
synthesis | the organism is used for production of 4-aminobutanoate, the enzyme is heterologously expressed in Escherichia coli, method optimization, overview | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
gene gadB, expression in Escherichia coli strain W3110 in glucose medium without the addition of glutamate. Addition of 0.1 mM pyridoxal 5'-phosphate enhances the production of 4-aminobutanoate, whereas Tween 40 is unnecessary for the fermentation productivity, different cultivation methods, overview | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Corynebacterium glutamicum | - |
4-aminobutanoate + CO2 | - |
? | |
L-glutamate | Corynebacterium glutamicum GAD | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene gadB | - |
Corynebacterium glutamicum GAD | - |
gene gadB | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimization of enzyme and 4-aminobutanoate production by recombinant enzyme heterologously expressed in Escherichia coli strain W3110, glucose medium without the addition of glutamate, addition of 0.1 mM pyridoxal 5'-phosphate enhances the production of 4-aminobutanoate, whereas Tween 40 is unnecessary for the fermentation productivity, different cultivation methods, overview | Corynebacterium glutamicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Corynebacterium glutamicum | 4-aminobutanoate + CO2 | - |
? | |
L-glutamate | - |
Corynebacterium glutamicum GAD | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GadB | - |
Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Corynebacterium glutamicum |