Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asiaticoside | 20% activation at 0.01 mg/ml | Homo sapiens | |
asiaticoside | an increase in GAD65 activity by 22% is observed with asiaticoside | Homo sapiens | |
asiaticoside | an increase in GAD65 activity by 4.5% is observed with asiaticoside | Homo sapiens | |
bilobalide | 20% activation at 0.01 mg/ml | Homo sapiens | |
bilobalide | an increase in GAD65 activity by approximately 18% is observed with bilobalide | Homo sapiens | |
bilobalide | an increase in GAD65 activity by 17.8% is observed with bilobalide | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
expressed in Escherichia coli DH5alpha cells | Homo sapiens |
gene gadB, expression in Escherichia coli strain DH5alpha using plasmids pMAL-c2X and the pGEX-3X and fusion to the maltose binding protein or GST, cytoplasmic targeting, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-pent-2-enoic acid | 20% inhibition at 0.01 mg/ml | Homo sapiens | |
3-Mercaptopropionic acid | 43% inhibition at 0.1 mM | Homo sapiens | |
allylglycine | - |
Homo sapiens | |
asiaticoside | - |
Homo sapiens | |
betulinic acid | 27% inhibition at 0.01 mg/ml | Homo sapiens | |
bilobalide | - |
Homo sapiens | |
ginsenosides | 23% inhibition at 0.01 mg/ml, ethanol extract of Panax quinquefolius, different glycoside derivatives, overview | Homo sapiens | |
additional information | aasiaticoside does not inhibit GAD activity even at the highest dose of 0.03 mg/ml. Allylglycine does not inhibit GAD activity even at the highest dose of 0.1 mM; asiaticoside does not inhibit GAD activity even at the highest dose of 0.03 mg/ml, allylglycine exhibits no enzyme inhibition even at the highest dose of 0.1 mM; minor inhibition is seen with the ethanol extract of Panax quinquefolius (23%), betulinic acid (27%), and valerenic acid (20%); minor inhibition is seen with the ethanol extract of Panax quinquefolius (ginsenosides) (23%) | Homo sapiens | |
ursolic acid | 11.2% inhibition at 0.01 mg/ml | Homo sapiens | |
valerenic acid | 20% inhibition at 0.01 mg/ml | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Homo sapiens | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography, glutathione Sepharose 4B resin column chromatography | Homo sapiens |
glutathione sepharose 4B resin column chromatography and amylose resin column chromatography | Homo sapiens |
recombinant tagged enzyme from Escherichia coli strain DH5alpha | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nervous system | two isoforms ofGAD, GAD67and GAD65 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Homo sapiens | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GAD65 | - |
Homo sapiens |
GAD65 | isoform | Homo sapiens |
glutamic acid decarboxylase 65 | - |
Homo sapiens |
glutamic acid decarboxylase 65 | isoform | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens | |
pyridoxal 5'-phosphate | dependent on | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0123 | - |
- |
Homo sapiens | 3-Mercaptopropionic acid | |
0.0123 | - |
at 37°C | Homo sapiens | 3-Mercaptopropionic acid | |
0.0123 | - |
at 37°C, pH not specified in the publication | Homo sapiens | 3-Mercaptopropionic acid |