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Literature summary for 4.1.1.15 extracted from

  • Buss, K.; Drewke, C.; Lohmann, S.; Piwonska, A.; Leistner, E.
    Properties and interaction of heterologously expressed glutamate decarboxylase isoenzymes GAD(65kDa) and GAD(67kDa) from human brain with ginkgotoxin and its 5'-phosphate (2001), J. Med. Chem., 44, 3166-3174.
    View publication on PubMed
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
6*His-tagged isoforms GAD65 and GAD67 expressed in Saccharomyces cerevisiae Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4'-deoxypyridoxine-5'-phosphate about 60% inhibition of GAD65 at 3.5 mM, GAD67 is hardly affected Homo sapiens
4'-O-methylpyridoxine-5'-phosphate decreases activity of GAD65 in unphysiologically high concentrations, about 60% inhibition at 3.5 mM. GAD67 activity is hardly affected Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 L-Glu 6*His-tagged GAD67, crude extract Homo sapiens
1.3
-
 L-Glu GAD65 after cleavage with protease factor Xa Homo sapiens
1.4
-
 L-Glu 6*His-tagged GAD65 Homo sapiens
1.4
-
 L-Glu GAD67 after cleavage with protease factor Xa Homo sapiens
2.5
-
 L-Glu GAD67-GST fusion protein expressed in Escherichia coli Homo sapiens
2.9
-
 L-Glu GAD65-GST fusion protein expressed in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoform GAD65 and GAD67 expressed in Escherichia coli as fusion protein with glutathione-S-transferase and 6*His-tagged isoforms GAD65 and GAD67 expressed in Saccharomyces cerevisiae Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Glu
-
 Homo sapiens 4-Aminobutanoate + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42 GAD65-GST fusion protein expressed in Escherichia coli Homo sapiens
39 43 6*His-tagged GAD65 Homo sapiens
39 43 GAD65 after cleavage with protease factor Xa Homo sapiens
43 50 6*His-tagged GAD67, crude extract Homo sapiens
43 50 GAD67-GST fusion protein expressed in Escherichia coli Homo sapiens
45 50 GAD67 after cleavage with protease factor Xa Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 L-Glu GAD65 after cleavage with protease factor Xa Homo sapiens
1.8
-
 L-Glu 6*His-tagged GAD65 Homo sapiens
1.8
-
 L-Glu GAD65-GST fusion protein expressed in Escherichia coli Homo sapiens
6.2
-
 L-Glu GAD67 after cleavage with protease factor Xa Homo sapiens
7.7
-
 L-Glu GAD67-GST fusion protein expressed in Escherichia coli Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activity of GST-GAD54, GAD65 and 6*His-GAD65 is nearly zero without exogenous pyridoxal 5'-phosphate. Activity of GST-GAD67 is 20% without exogenous pyridoxal 5'-phosphate. Activity of GAD67 is 32% without exogenous pyridoxal 5'-phosphate. Activity of 6*His-GAD67 is 30% without exogenous pyridoxal 5'-phosphate Homo sapiens