Cloned (Comment) | Organism |
---|---|
creation of a hybrid form of GAD consisting of amino acids 1-101 of the human GAD67 protein fused to amino acids 96-585 of the human GAD65 protein, and modification of this to include a C-terminal hexa-His tag sequence. The hybrid GAD67/65-H6 is expressed in two yeast hosts: constitutively under the control of the plasmid oxidase promoter PGK1 in Saccharomyces cerevisiae, and inducibly under the control of the chromosomal alcohol oxidase promoter AOX1 in Pichia pastoris. The hybrid GAD67/65 is isolated at high specific activity and moderate yield, and the addition of the His6 tag sequence of the choice of the yeast strain does not appreciably affect enzyme activity, percentage recovery of GAD, protein purification or utility in diagnosis of diabetes in terms of specificity and sensitivity to the various sera | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | creation of a hybrid form of GAD consisting of amino acids 1-101 of the human GAD67 protein fused to amino acids 96-585 of the human GAD65 protein, and modification of this to include a C-terminal hexa-His tag sequence. The hybrid GAD67/65-H6 is expressed in two yeast hosts: consitutively under the control of the plasmid oxidase promoter PGK1 in Saccharomyces cerevisiae, and inducibly under the control of the chromosomal alcohol oxidase promoter AOX1 in Pichia pastoris. The hybrid GAD67/65 is isolated at high specific activity and moderate yield, and the addition of the His6 tag sequence of the choice of the yeast strain does not appreciably affect enzyme activity, percentage recovery of GAD, protein purification or utility in diagnosis of diabetes in terms of specificity and sensitivity to the various sera | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.956 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Glu | - |
Homo sapiens | 4-Aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAD | - |
Homo sapiens |