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Literature summary for 4.1.1.12 extracted from
- Expression, purification, and biochemical characterization of Mycobacterium tuberculosis aspartate decarboxylase, PanD (2002), Protein Expr. Purif., 25, 533-540.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
L-aspartate | 37°C, pH 7.0 | Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
4 * 16000, SDS-PAGE, PanD is expressed as 16000 Da pro-protein that is processed into 1 alpha and 1 beta subunit | Mycobacterium tuberculosis |
| 64000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | Mycobacterium tuberculosis | - |
L-alanine + CO2 | - |
? | |
| L-aspartate | Mycobacterium tuberculosis H37Rv | - |
L-alanine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant panD | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.1 | - |
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | - |
Mycobacterium tuberculosis | L-alanine + CO2 | - |
? | |
| L-aspartate | - |
Mycobacterium tuberculosis H37Rv | L-alanine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 16000, SDS-PAGE, PanD is expressed as 16000 Da pro-protein that is processed into 1 alpha and 1 beta subunit | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PanD | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
L-aspartate | 37°C, pH 7.0 | Mycobacterium tuberculosis | |
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