BRENDA - Enzyme Database
show all sequences of 4.1.1.12

Properties of crystalline L-aspartate beta-decarboxylase of Pseudomonas dacunhae

Shibatani, T.; Kakimoto, T.; Kato, J.; Nishimura, N.; Chibata, I.; J. Ferment. Technol. 52, 886-898 (1974)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-oxoglutarate
activates
Pseudomonas dacunhae
DL-erythro-3-hydroxyaspartate
-
Pseudomonas dacunhae
DL-threo-3-hydroxyaspartate
-
Pseudomonas dacunhae
oxalacetate
activates
Pseudomonas dacunhae
Crystallization (Commentary)
Crystallization
Organism
-
Pseudomonas dacunhae
Inhibitors
Inhibitors
Commentary
Organism
Structure
fumarate
-
Pseudomonas dacunhae
L-cysteine sulfinate
-
Pseudomonas dacunhae
malate
-
Pseudomonas dacunhae
Maleate
-
Pseudomonas dacunhae
malonate
-
Pseudomonas dacunhae
succinate
-
Pseudomonas dacunhae
Tartrate
-
Pseudomonas dacunhae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
L-Asp
in presence of 2-oxoglutarate or pyridoxal 5'-phosphate
Pseudomonas dacunhae
5
-
L-Asp
in absence of cofactors
Pseudomonas dacunhae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas dacunhae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Asp
highly specific for L-Asp
3958
Pseudomonas dacunhae
L-Ala + CO2
-
3958
Pseudomonas dacunhae
-
L-Cysteinesulfinate
-
3958
Pseudomonas dacunhae
L-Ala + SO2
-
-
-
-
additional information
transamination between Asp and 2-oxoglutarate
3958
Pseudomonas dacunhae
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
47
-
decarboxylation and transamination
Pseudomonas dacunhae
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
40
20°C: about 60% of maximal activity, 40°C: about 20% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
30
50
30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, aspartate decarboxylase activity
Pseudomonas dacunhae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5
-
transamination between Asp and 2-oxoglutarate
Pseudomonas dacunhae
4
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
5.3
-
aspartate decarboxylase activity
Pseudomonas dacunhae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3
5
pH 3: about 35% of maximal activity, pH 5: about 40% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4
6
about 20% of maximal activity at pH 4 and pH 6, aspartate decarboxylase activity
Pseudomonas dacunhae
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
activates
Pseudomonas dacunhae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-oxoglutarate
activates
Pseudomonas dacunhae
DL-erythro-3-hydroxyaspartate
-
Pseudomonas dacunhae
DL-threo-3-hydroxyaspartate
-
Pseudomonas dacunhae
oxalacetate
activates
Pseudomonas dacunhae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
activates
Pseudomonas dacunhae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Pseudomonas dacunhae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fumarate
-
Pseudomonas dacunhae
L-cysteine sulfinate
-
Pseudomonas dacunhae
malate
-
Pseudomonas dacunhae
Maleate
-
Pseudomonas dacunhae
malonate
-
Pseudomonas dacunhae
succinate
-
Pseudomonas dacunhae
Tartrate
-
Pseudomonas dacunhae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
L-Asp
in presence of 2-oxoglutarate or pyridoxal 5'-phosphate
Pseudomonas dacunhae
5
-
L-Asp
in absence of cofactors
Pseudomonas dacunhae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Asp
highly specific for L-Asp
3958
Pseudomonas dacunhae
L-Ala + CO2
-
3958
Pseudomonas dacunhae
-
L-Cysteinesulfinate
-
3958
Pseudomonas dacunhae
L-Ala + SO2
-
-
-
-
additional information
transamination between Asp and 2-oxoglutarate
3958
Pseudomonas dacunhae
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
47
-
decarboxylation and transamination
Pseudomonas dacunhae
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
40
20°C: about 60% of maximal activity, 40°C: about 20% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
30
50
30°C: about 50% of maximal activity, 50°C: about 65% of maximal activity, aspartate decarboxylase activity
Pseudomonas dacunhae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5
-
transamination between Asp and 2-oxoglutarate
Pseudomonas dacunhae
4
-
cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
5.3
-
aspartate decarboxylase activity
Pseudomonas dacunhae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3
5
pH 3: about 35% of maximal activity, pH 5: about 40% of maximal activity, cysteine sulfinate desulfinase activity
Pseudomonas dacunhae
4
6
about 20% of maximal activity at pH 4 and pH 6, aspartate decarboxylase activity
Pseudomonas dacunhae
Other publictions for EC 4.1.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714193
Phillips
Insights into the mechanism of ...
Pseudomonas dacunhae
Biochemistry
49
5066-5073
2010
-
-
-
-
1
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2
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4
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1
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-
1
-
1
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-
-
4
-
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-
-
-
-
-
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-
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-
-
-
-
705144
Lima
The crystal structure of the P ...
Pseudomonas dacunhae
J. Mol. Biol.
388
98-108
2009
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-
1
1
2
-
-
2
-
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2
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1
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1
1
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2
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1
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1
1
1
2
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2
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1
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-
1
1
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-
2
-
-
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705636
Richardson
An examination of aspartate de ...
Aedes aegypti
Mol. Biol. Rep.
37
3199-3205
2009
-
-
1
-
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-
1
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1
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1
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1
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2
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2
1
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1
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1
1
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1
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1
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1
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2
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2
1
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-
-
706788
Chen
Structure, assembly, and mecha ...
Comamonas testosteroni, Comamonas testosteroni CCRC 11585, Pseudomonas sp.
Structure
17
517-529
2009
-
-
2
2
12
-
-
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-
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3
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2
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-
3
2
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7
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2
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2
2
2
12
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-
2
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-
-
3
2
-
-
-
7
-
-
-
-
-
-
-
-
-
-
690741
Wang
Inactive S298R disassembles th ...
Pseudomonas sp.
Biochem. Biophys. Res. Commun.
374
134-137
2008
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
1
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-
2
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1
1
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1
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3
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1
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2
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1
1
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671797
Wang
Enhanced transaminase activity ...
Pseudomonas sp.
Biochem. Biophys. Res. Commun.
356
368-373
2007
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1
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7
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1
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1
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3
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2
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1
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1
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1
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1
1
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7
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1
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3
-
2
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1
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-
1
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-
-
-
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-
677736
Wang
Molecular cloning of the aspar ...
Pseudomonas sp.
Appl. Microbiol. Biotechnol.
73
339-348
2006
3
2
1
-
-
-
9
1
-
1
3
1
-
4
-
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1
-
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-
1
-
3
2
1
1
-
-
1
-
1
1
-
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-
3
2
1
1
-
-
-
-
9
-
1
-
1
3
1
-
-
-
1
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-
1
-
3
2
1
1
-
-
1
-
1
-
-
-
-
-
-
-
653749
Chopra
Expression, purification, and ...
Mycobacterium tuberculosis
Protein Expr. Purif.
25
533-540
2002
-
-
1
-
-
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-
1
-
-
2
2
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1
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1
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1
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2
1
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1
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1
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1
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2
2
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1
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1
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2
1
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1
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-
652688
Chen
-
Cloning, expression and charac ...
Comamonas testosteroni
J. Ind. Microbiol. Biotechnol.
25
132-140
2000
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1
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-
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2
1
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1
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1
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-
2
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1
1
1
1
-
-
1
1
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-
1
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-
1
-
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-
-
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-
-
2
1
-
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-
1
-
-
2
-
1
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
3965
El-Rahmany
Comparison of L-aspartate 4-ca ...
Cunninghamella elegans, Penicillium citrinum
Microbiol. Res.
149
253-157
1994
6
-
-
-
-
-
8
2
-
8
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-
2
-
-
-
-
-
-
-
4
2
-
2
2
2
-
2
2
-
2
-
-
-
6
-
-
2
-
-
-
-
8
-
2
-
8
-
-
-
-
-
-
-
-
-
4
2
-
2
2
2
-
2
2
-
-
-
-
-
-
-
-
3964
Abelian
Intracellular L-aspartate-beta ...
Alcaligenes sp., Pseudomonas sp.
Biokhimiya
56
1288-1295
1991
-
-
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2
2
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2
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2
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2
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2
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2
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2
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2
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2
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3963
Takamatsu
Recirculating bioreactor-separ ...
Pseudomonas dacunhae
Biotechnol. Bioeng.
32
184-191
1988
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1
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1
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3960
Rathod
Regulation of mammalian aspart ...
Rattus norvegicus
Arch. Biochem. Biophys.
238
447-451
1985
1
-
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-
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-
1
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1
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1
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1
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2
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1
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1
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1
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1
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2
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3961
Wong
Rat brain aspartate beta-decar ...
Rattus norvegicus
Neurochem. Int.
7
351-355
1985
1
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8
2
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1
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3
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1
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2
1
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1
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1
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1
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8
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2
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3
-
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1
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2
1
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-
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3962
Rathod
Identification of mammalian as ...
Bos taurus, Felis catus, Gallus gallus, Mus musculus, Rattus norvegicus, Sus scrofa
Arch. Biochem. Biophys.
238
435-446
1985
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2
1
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11
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1
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9
1
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13
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1
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2
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2
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1
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1
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9
1
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13
-
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1
-
2
-
-
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-
-
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3966
Rosenberg
Aspartate beta-decarboxylase f ...
Alcaligenes faecalis
Biochemistry
24
1598-1603
1985
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2
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1
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659615
Furui
-
Pressurized reaction method fo ...
Pseudomonas dacunhae
J. Ferment. Technol.
61
587-591
1983
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1
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1
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1
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-
1
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1
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-
1
-
-
-
-
-
-
-
-
-
3967
Chang
Some stereochemical features o ...
Alcaligenes faecalis
J. Biol. Chem.
257
3564-3569
1982
-
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1
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1
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1
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3959
Takamatsu
-
Stabilization of L-aspartate b ...
Pseudomonas dacunhae
J. Ferment. Technol.
59
489-493
1981
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1
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1
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1
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1
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1
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3956
Relyea
Affinity labeling of the activ ...
Alcaligenes faecalis
J. Biol. Chem.
249
1519-1524
1974
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1
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1
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1
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3957
Ikeda
Studies of the activity of sub ...
Pseudomonas dacunhae
Eur. J. Biochem.
46
553-558
1974
1
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1
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1
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1
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1
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1
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1
1
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3958
Shibatani
-
Properties of crystalline L-as ...
Pseudomonas dacunhae
J. Ferment. Technol.
52
886-898
1974
4
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1
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2
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1
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3
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2
2
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3
2
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1
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4
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1
1
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7
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2
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3
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2
2
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3
2
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3955
Tate
L-Aspartate-beta-decarboxylase ...
Acetobacter sp., Achromobacter sp., Alcaligenes faecalis, Astacoidea, Bombyx mori, Clostridium perfringens, Desulfovibrio desulfuricans, Lobster, Nocardia globerula, Pseudomonas dacunhae, Pseudomycobacteria
Adv. Enzymol. Relat. Areas Mol. Biol.
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503-543
1971
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6
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11
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19
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4
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18
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4
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6
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19
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3952
Palekar
Inhibition of aspartate beta-d ...
Alcaligenes faecalis
Biochemistry
9
2310-2315
1970
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1
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2
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3954
Kakimoto
Crystalline L-aspartate beta-d ...
Pseudomonas dacunhae
J. Biol. Chem.
244
353-358
1969
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1
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2
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2
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1
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1
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1
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1
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1
1
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2
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1
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1
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1
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3953
Novogrodsky
Control of aspartate beta-deca ...
Alcaligenes faecalis
J. Biol. Chem.
239
879-888
1964
1
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1
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1
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1
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1
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1
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2
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3951
Wilson
Properties of crystalline L-as ...
Achromobacter sp.
Biochem. J.
88
578-587
1963
6
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1
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1
9
1
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1
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1
1
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1
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2
1
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6
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1
1
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1
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9
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1
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1
1
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1
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