BRENDA - Enzyme Database
show all sequences of 4.1.1.112

Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis

Espariz, M.; Repizo, G.; Blancato, V.; Mortera, P.; Alarcon, S.; Magni, C.; FEBS J. 278, 2140-2151 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Enterococcus faecalis
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
78% residual activity at 0.25 mM
Enterococcus faecalis
ATP
37% residual activity at 1 mM
Enterococcus faecalis
citrate
74% residual activity at 2 mM
Enterococcus faecalis
EDTA
complete inhibition at 2 mM
Enterococcus faecalis
malate
65% residual activity at 2 mM
Enterococcus faecalis
malonate
26% residual activity at 2 mM
Enterococcus faecalis
additional information
not inhibited by succinate
Enterococcus faecalis
NAD+
40% residual activity at 0.25 mM
Enterococcus faecalis
NADH
53% residual activity at 0.25 mM
Enterococcus faecalis
oxalate
14% residual activity at 2 mM
Enterococcus faecalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.62
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+
Enterococcus faecalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterococcus faecalis
Q82YW6
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-bounded affinity column chromatography
Enterococcus faecalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
-
714979
Enterococcus faecalis
Pyruvate + CO2
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.2
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Enterococcus faecalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
5
-
Enterococcus faecalis
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no nicotinamide cofactors required
Enterococcus faecalis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Enterococcus faecalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no nicotinamide cofactors required
Enterococcus faecalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
78% residual activity at 0.25 mM
Enterococcus faecalis
ATP
37% residual activity at 1 mM
Enterococcus faecalis
citrate
74% residual activity at 2 mM
Enterococcus faecalis
EDTA
complete inhibition at 2 mM
Enterococcus faecalis
malate
65% residual activity at 2 mM
Enterococcus faecalis
malonate
26% residual activity at 2 mM
Enterococcus faecalis
additional information
not inhibited by succinate
Enterococcus faecalis
NAD+
40% residual activity at 0.25 mM
Enterococcus faecalis
NADH
53% residual activity at 0.25 mM
Enterococcus faecalis
oxalate
14% residual activity at 2 mM
Enterococcus faecalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.62
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+
Enterococcus faecalis
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-bounded affinity column chromatography
Enterococcus faecalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
-
714979
Enterococcus faecalis
Pyruvate + CO2
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.2
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Enterococcus faecalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
5
-
Enterococcus faecalis
General Information
General Information
Commentary
Organism
malfunction
the citrate fermentation phenotype is not affected by citM deletion
Enterococcus faecalis
metabolism
CitM is not required for efficient citrate utilization
Enterococcus faecalis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the citrate fermentation phenotype is not affected by citM deletion
Enterococcus faecalis
metabolism
CitM is not required for efficient citrate utilization
Enterococcus faecalis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
22.3
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
22.3
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
Other publictions for EC 4.1.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748206
Pircher
Identification of FAH domain- ...
Homo sapiens
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2015
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726821
Lietzan
Functionally diverse biotin-de ...
Klebsiella aerogenes, Vibrio cholerae
Arch. Biochem. Biophys.
544
75-86
2014
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3
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5
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726737
Repizo
Biochemical and genetic charac ...
Enterococcus faecalis, Enterococcus faecalis JH2-2
Appl. Environ. Microbiol.
79
2882-2890
2013
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1
1
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713674
Ran
Expression, purification, crys ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
67
968-970
2011
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1
1
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1
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714979
Espariz
Identification of malic and so ...
Enterococcus faecalis
FEBS J.
278
2140-2151
2011
-
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1
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10
1
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1
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1
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1
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1
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1
1
1
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1
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1
1
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10
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1
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1
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1
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1
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1
1
1
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2
2
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1
1
715662
Balsera
Quaternary structure of the ox ...
Vibrio cholerae
J. Biol. Chem.
286
9457-9467
2011
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1
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1
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1
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704327
Klaffl
Genetic and functional analysi ...
Corynebacterium glutamicum
J. Bacteriol.
192
2604-2612
2010
-
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1
1
1
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3
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2
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1
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1
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1
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1
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1
1
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716673
Granjon
Structure-function relations i ...
Vibrio cholerae
PLoS ONE
5
e10935
2010
-
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1
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2
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1
1
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1
1
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1
1
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678331
Narayanan
Structure and function of PA48 ...
Pseudomonas aeruginosa
Biochemistry
47
167-182
2008
-
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1
1
3
-
6
5
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1
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3
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1
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1
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2
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3
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6
6
5
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1
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2
1
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2
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692317
Dharmarajan
Tyr235 of human cytosolic phos ...
Homo sapiens
FEBS J.
275
5810-5819
2008
-
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1
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3
-
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2
1
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1
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1
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1
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1
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3
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2
1
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1
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1
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692806
Augagneur
Mutation of the oxaloacetate d ...
Lactococcus lactis
J. Appl. Microbiol.
104
260-268
2008
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4
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692884
Blancato
Transcriptional regulation of ...
Enterococcus faecalis
J. Bacteriol.
190
7419-7430
2008
1
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1
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1
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681424
Studer
Crystal structure of the carbo ...
Vibrio cholerae
J. Mol. Biol.
367
547-557
2007
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1
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10
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1
1
3
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1
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1
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1
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10
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3
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1
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1
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679954
Kim
-
Determining citrate in fruit j ...
Pseudomonas sp.
Food Chem.
99
851-857
2006
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1
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663796
Dahinden
Oxaloacetate decarboxylase of ...
Vibrio cholerae, Vibrio cholerae O395-N1
Arch. Microbiol.
183
121-129
2005
-
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1
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-
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2
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1
1
2
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16
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1
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1
1
1
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1
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1
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664904
Dahinden
Identification of a domain in ...
Vibrio cholerae
FEBS J.
272
846-855
2005
-
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1
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1
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663795
Dahinden
Oxaloacetate decarboxylase of ...
Archaeoglobus fulgidus
Arch. Microbiol.
182
414-420
2004
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1
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664936
Sender
Characterization of an oxaloac ...
Lactococcus lactis, Lactococcus lactis CRL264
FEBS Lett.
570
217-222
2004
-
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1
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15
1
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3
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5
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1
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2
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1
1
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2
1
1
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1
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15
-
1
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3
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1
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2
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1
1
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2
1
1
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-
650185
Wild
Site-directed sulfhydryl label ...
Klebsiella pneumoniae
Biochemistry
42
11615-11624
2003
-
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1
-
6
-
-
-
1
1
-
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
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1
1
-
6
-
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1
1
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1
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1
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2
1
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651122
Schmid
Role of conserved residues wit ...
Klebsiella pneumoniae
Eur. J. Biochem.
269
2997-3004
2002
-
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1
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7
-
1
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1
2
-
1
-
2
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1
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2
1
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3
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1
2
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1
1
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7
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1
2
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1
2
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1
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1
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2
1
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3
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-
653877
Trukhina
-
Subcellular localization of ox ...
Zea mays
Russ. J. Plant Physiol.
49
635-640
2002
2
-
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1
1
2
2
1
1
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1
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1
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1
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