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Literature summary for 4.1.1.112 extracted from

  • Wild, M.R.; Pos, K.M.; Dimroth, P.
    Site-directed sulfhydryl labeling of the oxaloacetate decarboxylase Na+ pump of Klebsiella pneumoniae: helix VIII comprises a portion of the sodium ion channel (2003), Biochemistry, 42, 11615-11624.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
A383C beta subunit, very low activity, low activity after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
G377C beta subunit, no activity even after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
G380C beta subunit, very low activity, low activity after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
additional information several other mutant with reduced activity Klebsiella pneumoniae
R389C beta subunit, increased pH-optimum, reduced activity, activity and pH-optimum can be restored to wild type value by modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae
S382C beta subunit, no activity even after modification with 2-aminoethyl methanethiosulfonate Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate
-
Klebsiella pneumoniae Pyruvate + CO2
-
?
Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
?

Subunits

Subunits Comment Organism
trimer alpha, beta, gamma Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae