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Literature summary for 4.1.1.112 extracted from
- Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum (1995), Antonie van Leeuwenhoek, 67, 221-227.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | optimization of oxaloacetate may increase lysine productivity of commercially used strains | Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | Ki: 1.2 mM | Corynebacterium glutamicum |  |
| Avidin | not | Corynebacterium glutamicum | |
| coenzyme A | Ki: 2.4 mM | Corynebacterium glutamicum | |
| Cu2+ | Ki: 0.2 mM | Corynebacterium glutamicum | |
| succinate | - |
Corynebacterium glutamicum | |
| Zn2+ | Ki: 0.8 mM | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
oxaloacetate | - |
Corynebacterium glutamicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Micrococcus luteus | |
| Mn2+ | Km: 1.2 mM | Corynebacterium glutamicum | |
| Mn2+ | required, can be substituted by Mg2+, Co2+, Ni2+, Ca2+ | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31700 | - |
4 * 31700, SDS-PAGE | Corynebacterium glutamicum |
| 118000 | - |
gel filtration | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate | Corynebacterium glutamicum | - |
? | - |
? | |
| oxaloacetate | Micrococcus luteus | - |
? | - |
? | |
| oxaloacetate | Azotobacter vinelandii | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
| Corynebacterium glutamicum | - |
- |
- |
| Micrococcus luteus | - |
formerly Micrococcus lysodeiktikus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
strain-dependend | Corynebacterium glutamicum |
| 158 | - |
- |
Corynebacterium glutamicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, potassium phosphate buffer, pH 7, 50% glycerol, 2 month | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Oxaloacetate | - |
Corynebacterium glutamicum | Pyruvate + CO2 | - |
? | |
| Oxaloacetate | - |
Micrococcus luteus | Pyruvate + CO2 | - |
? | |
| Oxaloacetate | - |
Azotobacter vinelandii | Pyruvate + CO2 | - |
? | |
| oxaloacetate | - |
Corynebacterium glutamicum | ? | - |
? | |
| oxaloacetate | - |
Micrococcus luteus | ? | - |
? | |
| oxaloacetate | - |
Azotobacter vinelandii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 31700, SDS-PAGE | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | not | Corynebacterium glutamicum | |
| biotin | not | Micrococcus luteus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Cu2+ | - |
Corynebacterium glutamicum | |
| 0.8 | - |
Zn2+ | - |
Corynebacterium glutamicum | |
| 2.4 | - |
coenzyme A | - |
Corynebacterium glutamicum | |
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