Cloned (Comment) | Organism |
---|---|
gene panD, subcloning and expression in strain DH5alpha, BL21(DE3), and in an enzyme-deficient strain, functional expression under the constitutive CaMV 35S promoter in transgenic Nicotiana tabacum cv. Havana 38 leaves using the Agrobacterium tumefaciens transfection system | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the expression of the enzyme in transgenic Nicotiana tabacum cv. Havana 38 leads to increased beta-alanine and pantothenate levels and improved thermotolerance in the tobacco plants, growth of homozygous lines expressing the bacterial enzyme is less affected than that of the control lines when the plants are stressed for 1 week at 35°C, tobacco seed germination at 42C is improved, phenotype,overview | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13800 | - |
x * 13800, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Escherichia coli | - |
beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene panD | - |
Purification (Comment) | Organism |
---|---|
recomnbinant enzyme from strain BL21(DE3) by anion exchange chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Escherichia coli | beta-alanine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 13800, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Escherichia coli |
L-Aspartate-alpha-decarboxylase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermotolerance of transgenic tobacco plants expressing the enzyme, overview | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |