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Literature summary for 4.1.1.11 extracted from

  • Fouad, W.M.; Rathinasabapathi, B.
    Expression of bacterial L-aspartate-alpha-decarboxylase in tobacco increases beta-alanine and pantothenate levels and improves thermotolerance (2006), Plant Mol. Biol., 60, 495-505.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene panD, subcloning and expression in strain DH5alpha, BL21(DE3), and in an enzyme-deficient strain, functional expression under the constitutive CaMV 35S promoter in transgenic Nicotiana tabacum cv. Havana 38 leaves using the Agrobacterium tumefaciens transfection system Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the expression of the enzyme in transgenic Nicotiana tabacum cv. Havana 38 leads to increased beta-alanine and pantothenate levels and improved thermotolerance in the tobacco plants, growth of homozygous lines expressing the bacterial enzyme is less affected than that of the control lines when the plants are stressed for 1 week at 35°C, tobacco seed germination at 42C is improved, phenotype,overview Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13800
-
x * 13800, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate Escherichia coli
-
beta-alanine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene panD
-

Purification (Commentary)

Purification (Comment) Organism
recomnbinant enzyme from strain BL21(DE3) by anion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
Escherichia coli beta-alanine + CO2
-
?

Subunits

Subunits Comment Organism
? x * 13800, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
ADC
-
Escherichia coli
L-Aspartate-alpha-decarboxylase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermotolerance of transgenic tobacco plants expressing the enzyme, overview Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Escherichia coli