Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by pyridoxal 5'-phosphate or thiamin diphosphate | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 100% loss of activity | Agrobacterium tumefaciens | |
Cu2+ | 1 mM, 26% loss of activity | Agrobacterium tumefaciens | |
Hg2+ | 1 mM, 47% loss of activity | Agrobacterium tumefaciens | |
additional information | not inhibitory: FeCl3, FeSO4, CoCl2, MgSO4, MnCl2, NiCl2, CaCl2, ZnSO4, EDTA, Tiron, 2,2?-bipyridyl, o-phenanthroline, 8-hydroxyquinoline, phenylhydrazine, hydroxylamine, iodoacetate, N-ethylmaleimide or NaBH4 | Agrobacterium tumefaciens | |
p-chloromercuribenzoate | 1 mM, 20% loss of activity | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
2,6-dihydroxybenzoate | pH 7.0, 30°C | Agrobacterium tumefaciens | |
0.12 | - |
2,3-Dihydroxybenzoate | pH 7.0, 30°C | Agrobacterium tumefaciens | |
25.9 | - |
1,3-dihydroxybenzene | pH 7.0, 30°C | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Agrobacterium tumefaciens |
138000 | - |
gel filtration | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens IAM12048 | - |
- |
- |
Storage Stability | Organism |
---|---|
4C, potassium phosphate buffer pH 7.0 containing 1 mM dithiothreitol, stable for 7 days | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dihydroxybenzene + CO2 | - |
Agrobacterium tumefaciens | 2,6-dihydroxybenzoate | in the presence of KHCO3, the enzyme catalyzes the regioselective carboxylation of 1,3-dihydroxybenzene into 2,6-dihydroxybenzoate at a molar conversion ratio of 30% | r | |
1,3-dihydroxybenzene + CO2 | - |
Agrobacterium tumefaciens IAM12048 | 2,6-dihydroxybenzoate | in the presence of KHCO3, the enzyme catalyzes the regioselective carboxylation of 1,3-dihydroxybenzene into 2,6-dihydroxybenzoate at a molar conversion ratio of 30% | r | |
2,3-dihydroxybenzoate | 146% of the activity with 2,6-dihydroxybenzoate | Agrobacterium tumefaciens | 1,2-dihydroxybenzene + CO2 | - |
r | |
2,3-dihydroxybenzoate | 146% of the activity with 2,6-dihydroxybenzoate | Agrobacterium tumefaciens IAM12048 | 1,2-dihydroxybenzene + CO2 | - |
r | |
2,6-dihydroxybenzoate | - |
Agrobacterium tumefaciens | 1,3-dihydroxybenzene + CO2 | - |
r | |
2,6-dihydroxybenzoate | - |
Agrobacterium tumefaciens IAM12048 | 1,3-dihydroxybenzene + CO2 | - |
r | |
additional information | no substrates: 2,4-dihydroxybenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, 2-hydroxybenzoate, 4-methoxybenzoate, 4-aminobenzoate, 2,4,6-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, benzoate and terephthalate | Agrobacterium tumefaciens | ? | - |
? | |
additional information | no substrates: 2,4-dihydroxybenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, 2-hydroxybenzoate, 4-methoxybenzoate, 4-aminobenzoate, 2,4,6-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, benzoate and terephthalate | Agrobacterium tumefaciens IAM12048 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, SDS-PAGE | Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Agrobacterium tumefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 18% loss of activity | Agrobacterium tumefaciens |
70 | - |
30 min, complete loss of activity | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Agrobacterium tumefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | more than 80% of maximum activtiy within | Agrobacterium tumefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required | Agrobacterium tumefaciens |