Literature summary for 4.1.1.103 extracted from

Matsui, T.; Yoshida, T.; Yoshimura, T.; Nagasawa, T.
Regioselective carboxylation of 1,3-dihydroxybenzene by 2,6-dihydroxybenzoate decarboxylase of Pandoraea sp. 12B-2 (2006), Appl. Microbiol. Biotechnol., 73, 95-102.

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Application

Application	Comment	Organism
synthesis	enzyme catalyzes the regioselective carboxylation of phenol, 1,2-dihydroxybenzene and 1,3-dihydroxybenzen to 4-hydroxybenzoate, 2,3-dihydroxybenzoate, and 2,6-dihydroxybenzoate, respectively. When the efficient production of 2,6-dihydroxybenzoate is optimized using whole cells of Pandoraea sp. 12B-2, the productivity of 2,6-dihydroxybenzoate tops out at 1.43 M. No formation of any other products is observed after the carboxylation reaction	Pandoraea sp.

Organism

Organism	UniProt	Comment	Textmining
Pandoraea sp.	-	-	-
Pandoraea sp. 12B-2	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-dihydroxybenzene + CO2	-	Pandoraea sp.	2,3-dihydroxybenzoate	enzyme catalyzes the regioselective carboxylation	r
1,2-dihydroxybenzene + CO2	-	Pandoraea sp. 12B-2	2,3-dihydroxybenzoate	enzyme catalyzes the regioselective carboxylation	r
1,3-dihydroxybenzene + CO2	-	Pandoraea sp.	2,6-dihydroxybenzoate	reaction is reversible. In the presence of 0.1 M KHCO3, 0.5 mM 2,6-dihydroxybenzoate is formed from 12.5 mM 1,3-dihydroxybenzene. With 3 M KHCO3, the molar conversion ratio of 1,3-dihydroxybenzene reaches its highest value (48%)	r
1,3-dihydroxybenzene + CO2	-	Pandoraea sp. 12B-2	2,6-dihydroxybenzoate	reaction is reversible. In the presence of 0.1 M KHCO3, 0.5 mM 2,6-dihydroxybenzoate is formed from 12.5 mM 1,3-dihydroxybenzene. With 3 M KHCO3, the molar conversion ratio of 1,3-dihydroxybenzene reaches its highest value (48%)	r
2,6-dihydroxybenzoate	-	Pandoraea sp.	1,3-dihydroxybenzene + CO2	-	r
2,6-dihydroxybenzoate	-	Pandoraea sp. 12B-2	1,3-dihydroxybenzene + CO2	-	r
Phenol + CO2	-	Pandoraea sp.	4-Hydroxybenzoate	enzyme catalyzes the regioselective carboxylation	r
Phenol + CO2	-	Pandoraea sp. 12B-2	4-Hydroxybenzoate	enzyme catalyzes the regioselective carboxylation	r

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Release 2023.1 (January 2023)