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Literature summary for 4.1.1.102 extracted from
- 4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase- and tyrosine ammonia lyase-expressing Streptomyces lividans (2015), Biores. Technol., 180, 59-65 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | for 4-vinylphenol production directly from cellulose, L-tyrosine ammonia lyase derived from Rhodobacter sphaeroides and phenolic acid decarboxylase from Streptomyces sviceus are introduced into endoglucanase-secreting Streptomyces lividans, and the 4-vinylphenol biosynthetic pathway is constructed therein. The created transformants successfully produce 4-vinylphenol directly from cellulose | Streptomyces sviceus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Streptomyces hygroscopicus |
| expression in Escherichia coli BL21 (DE3) | Streptomyces sviceus |
| expression in Escherichia coli BL21 (DE3) | Streptomyces cattleya |
| expression in Streptomyces lividans | Streptomyces sviceus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces cattleya |  |
| 0.74 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces sviceus | |
| 1.07 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces hygroscopicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces cattleya | F8JS69 | - |
- |
| Streptomyces hygroscopicus | - |
- |
- |
| Streptomyces sviceus | B5HL29 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces hygroscopicus |
- |
Streptomyces sviceus |
- |
Streptomyces cattleya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxycinnamic acid | - |
Streptomyces hygroscopicus | 4-vinylphenol + CO2 | - |
? | |
| 4-hydroxycinnamic acid | - |
Streptomyces sviceus | 4-vinylphenol + CO2 | - |
? | |
| 4-hydroxycinnamic acid | - |
Streptomyces cattleya | 4-vinylphenol + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAD | - |
Streptomyces hygroscopicus |
| PAD | - |
Streptomyces sviceus |
| PAD | - |
Streptomyces cattleya |
| phenolic acid decarboxylase | - |
Streptomyces hygroscopicus |
| phenolic acid decarboxylase | - |
Streptomyces sviceus |
| phenolic acid decarboxylase | - |
Streptomyces cattleya |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces hygroscopicus |
| 30 | - |
- |
Streptomyces sviceus |
| 30 | - |
- |
Streptomyces cattleya |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | 10°C: about 70% of maximal activity, 50°C: about 40% of maximal activity | Streptomyces sviceus |
| 10 | 30 | 10°C: about 39% of maximal activity, 30°C: maximal activity, 40°C: no activity | Streptomyces hygroscopicus |
| 20 | 50 | 20°C: about 70% of maximal activity, 50°C: about 30% of maximal activity | Streptomyces cattleya |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces cattleya | |
| 0.96 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces sviceus | |
| 14.1 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces hygroscopicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Streptomyces sviceus |
| 5 | 6 | - |
Streptomyces hygroscopicus |
| 5 | 6 | - |
Streptomyces cattleya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | pH 4.0: about 30% of maximal activity, pH 7.0: about 60% of maximal activity | Streptomyces cattleya |
| 4 | 7 | pH 4.0: maximal activity, pH 7.0: about 65% of maximal activity | Streptomyces sviceus |
| 5 | 7 | pH 5.0-6.0: maximal activity, pH 7.0: about 45% of maximal activity | Streptomyces hygroscopicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
inactivated at | Streptomyces hygroscopicus |
| 4 | - |
inactivated at | Streptomyces cattleya |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces hygroscopicus | |
| 0.35 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces cattleya | |
| 1.31 | - |
4-hydroxycinnamic acid | pH 5, 30°C | Streptomyces sviceus | |
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Release 2023.1 (January 2023)
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