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Literature summary for 4.1.1.102 extracted from

  • Huang H.-K, H.H.; Chen L.-F, C.L.; Tokashiki M, T.M.; Ozawa T, O.T.; Taira T, T.T.; Ito S, I.S.
    An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii (2012), AMB Express, 2, 4.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cysteic acid 1 mM, 151% of initial activity Meyerozyma guilliermondii
D-cysteine 1 mM, 150% of initial activity Meyerozyma guilliermondii
dithiothreitol 0.2-1 mM, slightly enhances decarboxylation of ferulic acid without affecteing decarboxylation of 4-coumarate Meyerozyma guilliermondii
DL-homoserine 1 mM, 160% of initial activity Meyerozyma guilliermondii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Meyerozyma guilliermondii

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of structure based on PDB entry 2GC9. Residue Met57 is located at the entrance of the pocket and in the immediate vicinity of possible catalytic residues Arg60 and Glu82. Residue Met103 is spatially more distant from the two catalytic residues and located deeper in the active-site pocket Meyerozyma guilliermondii

Protein Variants

Protein Variants Comment Organism
M57A almost complete loss of activity Meyerozyma guilliermondii
M57L mutation does not increase the ratio of decarboxylation activity toward ferulate to 4-coumarate Meyerozyma guilliermondii
M57T almost complete loss of activity Meyerozyma guilliermondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19828
-
x * 19828, calculated Meyerozyma guilliermondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumarate Meyerozyma guilliermondii
-
4-vinylphenol + CO2
-
?
4-coumarate Meyerozyma guilliermondii ATCC 9058
-
4-vinylphenol + CO2
-
?
ferulate Meyerozyma guilliermondii
-
4-vinylguaiacol + CO2
-
?
ferulate Meyerozyma guilliermondii ATCC 9058
-
4-vinylguaiacol + CO2
-
?

Organism

Organism UniProt Comment Textmining
Meyerozyma guilliermondii G3XHW5
-
-
Meyerozyma guilliermondii ATCC 9058 G3XHW5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.1
-
mutant M57L, substrate ferulate, 25°C, pH 6.0 Meyerozyma guilliermondii
378
-
wild-type, substrate ferulate, 25°C, pH 6.0 Meyerozyma guilliermondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumarate
-
Meyerozyma guilliermondii 4-vinylphenol + CO2
-
?
4-coumarate 104% of the activity with ferulate Meyerozyma guilliermondii 4-vinylphenol + CO2
-
?
4-coumarate
-
Meyerozyma guilliermondii ATCC 9058 4-vinylphenol + CO2
-
?
4-coumarate 104% of the activity with ferulate Meyerozyma guilliermondii ATCC 9058 4-vinylphenol + CO2
-
?
4-hydroxycinnamate
-
Meyerozyma guilliermondii 4-hydroxystyrene + CO2
-
?
4-hydroxycinnamate
-
Meyerozyma guilliermondii ATCC 9058 4-hydroxystyrene + CO2
-
?
caffeate
-
Meyerozyma guilliermondii 3,4-dihydroxystyrene + CO2 14% of the activity with ferulate ?
caffeate
-
Meyerozyma guilliermondii ATCC 9058 3,4-dihydroxystyrene + CO2 14% of the activity with ferulate ?
ferulate
-
Meyerozyma guilliermondii 4-vinylguaiacol + CO2
-
?
ferulate
-
Meyerozyma guilliermondii ATCC 9058 4-vinylguaiacol + CO2
-
?

Subunits

Subunits Comment Organism
? x * 19828, calculated Meyerozyma guilliermondii

Synonyms

Synonyms Comment Organism
phenolic acid decarboxylase
-
Meyerozyma guilliermondii

General Information

General Information Comment Organism
physiological function an endogenous amino thiol-like compound with a molecular weight greater than 1400 Da activates Meyerozyma guilliermondii