Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteic acid | 1 mM, 151% of initial activity | Meyerozyma guilliermondii | |
D-cysteine | 1 mM, 150% of initial activity | Meyerozyma guilliermondii | |
dithiothreitol | 0.2-1 mM, slightly enhances decarboxylation of ferulic acid without affecteing decarboxylation of 4-coumarate | Meyerozyma guilliermondii | |
DL-homoserine | 1 mM, 160% of initial activity | Meyerozyma guilliermondii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Meyerozyma guilliermondii |
Crystallization (Comment) | Organism |
---|---|
modeling of structure based on PDB entry 2GC9. Residue Met57 is located at the entrance of the pocket and in the immediate vicinity of possible catalytic residues Arg60 and Glu82. Residue Met103 is spatially more distant from the two catalytic residues and located deeper in the active-site pocket | Meyerozyma guilliermondii |
Protein Variants | Comment | Organism |
---|---|---|
M57A | almost complete loss of activity | Meyerozyma guilliermondii |
M57L | mutation does not increase the ratio of decarboxylation activity toward ferulate to 4-coumarate | Meyerozyma guilliermondii |
M57T | almost complete loss of activity | Meyerozyma guilliermondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19828 | - |
x * 19828, calculated | Meyerozyma guilliermondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumarate | Meyerozyma guilliermondii | - |
4-vinylphenol + CO2 | - |
? | |
4-coumarate | Meyerozyma guilliermondii ATCC 9058 | - |
4-vinylphenol + CO2 | - |
? | |
ferulate | Meyerozyma guilliermondii | - |
4-vinylguaiacol + CO2 | - |
? | |
ferulate | Meyerozyma guilliermondii ATCC 9058 | - |
4-vinylguaiacol + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meyerozyma guilliermondii | G3XHW5 | - |
- |
Meyerozyma guilliermondii ATCC 9058 | G3XHW5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.1 | - |
mutant M57L, substrate ferulate, 25°C, pH 6.0 | Meyerozyma guilliermondii |
378 | - |
wild-type, substrate ferulate, 25°C, pH 6.0 | Meyerozyma guilliermondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumarate | - |
Meyerozyma guilliermondii | 4-vinylphenol + CO2 | - |
? | |
4-coumarate | 104% of the activity with ferulate | Meyerozyma guilliermondii | 4-vinylphenol + CO2 | - |
? | |
4-coumarate | - |
Meyerozyma guilliermondii ATCC 9058 | 4-vinylphenol + CO2 | - |
? | |
4-coumarate | 104% of the activity with ferulate | Meyerozyma guilliermondii ATCC 9058 | 4-vinylphenol + CO2 | - |
? | |
4-hydroxycinnamate | - |
Meyerozyma guilliermondii | 4-hydroxystyrene + CO2 | - |
? | |
4-hydroxycinnamate | - |
Meyerozyma guilliermondii ATCC 9058 | 4-hydroxystyrene + CO2 | - |
? | |
caffeate | - |
Meyerozyma guilliermondii | 3,4-dihydroxystyrene + CO2 | 14% of the activity with ferulate | ? | |
caffeate | - |
Meyerozyma guilliermondii ATCC 9058 | 3,4-dihydroxystyrene + CO2 | 14% of the activity with ferulate | ? | |
ferulate | - |
Meyerozyma guilliermondii | 4-vinylguaiacol + CO2 | - |
? | |
ferulate | - |
Meyerozyma guilliermondii ATCC 9058 | 4-vinylguaiacol + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19828, calculated | Meyerozyma guilliermondii |
Synonyms | Comment | Organism |
---|---|---|
phenolic acid decarboxylase | - |
Meyerozyma guilliermondii |
General Information | Comment | Organism |
---|---|---|
physiological function | an endogenous amino thiol-like compound with a molecular weight greater than 1400 Da activates | Meyerozyma guilliermondii |