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Literature summary for 4.1.1.1 extracted from

  • Buddrus, L.; Andrews, E.S.; Leak, D.J.; Danson, M.J.; Arcus, V.L.; Crennell, S.J.
    Crystal structure of pyruvate decarboxylase from Zymobacter palmae (2016), Acta Crystallogr. Sect. F, 72, 700-706.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zymobacter palmae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.15 M sodium citrate pH 5.5, 14% (w/v) PEG 3350 Zymobacter palmae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Zymobacter palmae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59400
-
 2 * 59400, calculated from amino acid sequence Zymobacter palmae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Zymobacter palmae
-
 acetaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Zymobacter palmae Q8KTX6
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Zymobacter palmae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Zymobacter palmae acetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
homotetramer 2 * 59400, calculated from amino acid sequence Zymobacter palmae

Synonyms

Synonyms Comment Organism
PDC
-
 Zymobacter palmae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Zymobacter palmae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the enzyme retains 80% activity after incubation at 65°C for 30 min Zymobacter palmae

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Zymobacter palmae