Literature summary for 4.1.1.1 extracted from

Krutsakorn, B.; Imagawa, T.; Honda, K.; Okano, K.; Ohtake, H.
Construction of an in vitro bypassed pyruvate decarboxylation pathway using thermostable enzyme modules and its application to N-acetylglutamate production (2013), Microb. Cell Fact., 12, 91.

Search for more literature for 4.1.1.1

Application

Application	Comment	Organism
synthesis	construction of a bypassed pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA, by using a coupled enzyme system consisting of pyruvate decarboxylase from Acetobacter pasteurianus and the CoA-acylating aldehyde dehydrogenase from Thermus thermophilus. A cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production is designed by coupling the bypassed pathway with the glutamate dehydrogenase from Thermus thermophilus and N-acetylglutamate synthase from Thermotoga maritima. N-Acetylglutamate can be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of a mixture of four recombinant Escherichia coli strains having either one of the thermostable enzymes. The overall recycling number of CoA is 27	Acetobacter pasteurianus
synthesis	construction of a bypassed pyruvate decarboxylation pathway, through which pyruvate can be converted to acetyl-CoA. The coupled enzyme system consists of pyruvate decarboxylase from Acetobacter pasteurianus and the CoA-acylating aldehyde dehydrogenase from Thermus thermophilus. A cofactor-balanced and CoA-recycling synthetic pathway for N-acetylglutamate production is established by coupling the bypassed pathway with the glutamate dehydrogenase from Thermus thermophilus and N-acetylglutamate synthase from Thermotoga maritima. N-Acetylglutamate can be produced from an equimolar mixture of pyruvate and alpha-ketoglutarate with a molar yield of 55% through the synthetic pathway consisting of a mixture of four recombinant Escherichia coli strains having either one of the thermostable enzymes. The overall recycling number of CoA is 27	Acetobacter pasteurianus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Acetobacter pasteurianus

Organism

Organism	UniProt	Comment	Textmining
Acetobacter pasteurianus	C7JF72	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.025	-	50°C, pH 8.5	Acetobacter pasteurianus
0.046	-	50°C, pH 6.0	Acetobacter pasteurianus
0.046	-	50°C, pH 8.0	Acetobacter pasteurianus
0.058	-	50°C, pH 7.5	Acetobacter pasteurianus
0.066	-	50°C, pH 6.5	Acetobacter pasteurianus
0.078	-	50°C, pH 7.0	Acetobacter pasteurianus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + CoA + NAD+	-	Acetobacter pasteurianus	acetyl-CoA + CO2 + NADH	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Acetobacter pasteurianus

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Release 2023.1 (January 2023)