Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Gluconacetobacter diazotrophicus |
Crystallization (Comment) | Organism |
---|---|
to 1.69 A resolution, monoclinic space group C2 with cell dimensions: a = 129.1 A, b =141.0 A, c = 91.1 A, beta = 125.8°, with two monomers per asymmetric unit | Gluconacetobacter diazotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
pyruvate | pH 5.0, 25°C | Gluconacetobacter diazotrophicus | |
0.06 | - |
pyruvate | at pH 5.0 and 25°C | Gluconacetobacter diazotrophicus | |
0.6 | - |
pyruvate | at pH 6.0 and 25°C | Gluconacetobacter diazotrophicus | |
1.2 | - |
pyruvate | pH 7.0, 25°C | Gluconacetobacter diazotrophicus | |
1.2 | - |
pyruvate | at pH 7.0 and 25°C | Gluconacetobacter diazotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Gluconacetobacter diazotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59200 | - |
- |
Gluconacetobacter diazotrophicus |
59200 | - |
x * 59200, calculated, x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
60000 | - |
x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
60000 | - |
x * 59200, calculated, x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Gluconacetobacter diazotrophicus | - |
acetaldehyde + CO2 | - |
? | |
pyruvate | Gluconacetobacter diazotrophicus ATCC 49037 | - |
acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconacetobacter diazotrophicus | A0A075Q354 | - |
- |
Gluconacetobacter diazotrophicus | A9H275 | - |
- |
Gluconacetobacter diazotrophicus ATCC 49037 | A9H275 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Gluconacetobacter diazotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
substrate 2-oxo-4-methylpentanoate, pH 5.0, 25°C | Gluconacetobacter diazotrophicus |
0.68 | - |
substrate 2-oxopentanoate, pH 5.0, 25°C | Gluconacetobacter diazotrophicus |
12 | - |
substrate 2-oxobutanoate, pH 5.0, 25°C | Gluconacetobacter diazotrophicus |
20 | - |
substrate pyruvate, pH 5.0, 25°C | Gluconacetobacter diazotrophicus |
43 | - |
substrate pyruvate, pH 7.0, 25°C | Gluconacetobacter diazotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Gluconacetobacter diazotrophicus | (S)-acetolactate + CO2 | - |
? | |
2-oxo-4-methylpentanoate | - |
Gluconacetobacter diazotrophicus | 3-methylbutanaldehyde + CO2 | - |
? | |
2-oxo-4-methylpentanoate | - |
Gluconacetobacter diazotrophicus | ? | - |
? | |
2-oxo-4-methylpentanoate | - |
Gluconacetobacter diazotrophicus ATCC 49037 | ? | - |
? | |
2-Oxobutanoate | - |
Gluconacetobacter diazotrophicus | ? | - |
? | |
2-Oxobutanoate | - |
Gluconacetobacter diazotrophicus ATCC 49037 | ? | - |
? | |
2-oxobutanoate | - |
Gluconacetobacter diazotrophicus | propionaldehyde + CO2 | - |
? | |
2-Oxopentanoate | - |
Gluconacetobacter diazotrophicus | ? | - |
? | |
2-Oxopentanoate | - |
Gluconacetobacter diazotrophicus ATCC 49037 | ? | - |
? | |
2-oxopentanoate | - |
Gluconacetobacter diazotrophicus | butanaldehyde + CO2 | - |
? | |
3-phenyl-2-oxopropanoate | - |
Gluconacetobacter diazotrophicus | ? | - |
? | |
additional information | no substrate: indole-3-pyruvate | Gluconacetobacter diazotrophicus | ? | - |
? | |
additional information | no activity with benzoylformate, 3-hydroxyphenylpyruvate and indole-3-pyruvate | Gluconacetobacter diazotrophicus | ? | - |
? | |
additional information | no activity with benzoylformate, 3-hydroxyphenylpyruvate and indole-3-pyruvate | Gluconacetobacter diazotrophicus ATCC 49037 | ? | - |
? | |
pyruvate | - |
Gluconacetobacter diazotrophicus | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Gluconacetobacter diazotrophicus ATCC 49037 | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
? | x * 59200, calculated, x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
? | x * 59200, calaculated from amino acid sequence | Gluconacetobacter diazotrophicus |
Synonyms | Comment | Organism |
---|---|---|
PDC | - |
Gluconacetobacter diazotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | - |
Gluconacetobacter diazotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life 18 min | Gluconacetobacter diazotrophicus |
60 | - |
the enzyme shows a half-life of18 min at 60°C | Gluconacetobacter diazotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Gluconacetobacter diazotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Gluconacetobacter diazotrophicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gluconacetobacter diazotrophicus | calculated | - |
5.8 |
Gluconacetobacter diazotrophicus | calaculated from amino acid sequence | - |
5.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
pyruvate | pH 7.0, 25°C | Gluconacetobacter diazotrophicus | |
140 | - |
pyruvate | at pH 7.0 and 25°C | Gluconacetobacter diazotrophicus | |
260 | - |
pyruvate | at pH 6.0 and 25°C | Gluconacetobacter diazotrophicus | |
1300 | - |
pyruvate | pH 5.0, 25°C | Gluconacetobacter diazotrophicus | |
1300 | - |
pyruvate | at pH 5.0 and 25°C | Gluconacetobacter diazotrophicus |