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Literature summary for 4.1.1.1 extracted from

  • Nguyen, T.; Drotar, A.M.; Monson, R.K.; Fall, R.
    A high affinity pyruvate decarboxylase is present in cottonwood leaf veins and petioles: a second source of leaf acetaldehyde emission? (2009), Phytochemistry, 70, 1217-1221.
    View publication on PubMed
Search for more literature for 4.1.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 pyruvate in 100 mM MES buffer (pH 5.6), 5 mM MgCl2, 1 mM dithiothreitol, and 1 mM thiamine diphosphate, at 25°C Populus deltoides
2.2
-
 2-ketobutyric acid in 100 mM MES buffer (pH 5.6), 5 mM MgCl2, 1 mM dithiothreitol, and 1 mM thiamine diphosphate, at 25°C Populus deltoides

Organism

Organism UniProt Comment Textmining
Populus deltoides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified, ammonium sulfate precipitation and phenyl Sepharose column chromatography Populus deltoides

Source Tissue

Source Tissue Comment Organism Textmining
leaf leaf vein Populus deltoides
-
petiole
-
 Populus deltoides
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 crude extract, at 25°C Populus deltoides
14.34
-
 after 143fold purification, at 25°C Populus deltoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketobutyric acid
-
 Populus deltoides propanal + CO2
-
 ?
pyruvate
-
 Populus deltoides acetaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
PDC
-
 Populus deltoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Populus deltoides