Protein Variants | Comment | Organism |
---|---|---|
E477Q/E91D | retains catalytic activity | Saccharomyces cerevisiae |
E51D/E91D | no residual catalytic activity | Saccharomyces cerevisiae |
E91D | racemic C2-alpha-lactylthiamine diphosphate exposed to mutant enzyme is partitioned between reversion to pyruvate and decarboxylation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2-oxo carboxylate = an aldehyde + CO2 | enzyme forms covalent intermediate C2-alpha-lactylthiamine diphosphate. Rate of reaction is limited by release of thiamine diphosphate | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | enzyme forms covalent intermediate C2-alpha-lactylthiamine diphosphate | Saccharomyces cerevisiae |