Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.1 extracted from

  • de la Plaza, M.; Fernandez de Palencia, P.; Pelaez, C.; Requena, T.
    Biochemical and molecular characterization of alpha-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis (2004), FEMS Microbiol. Lett., 238, 367-374.
    View publication on PubMed
Search for more literature for 4.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
NaCl up to 3fold increase in activity at pH 5.4 Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 95% inhibition Lactococcus lactis
Cu2+ 1 mM, 95% inhibition Lactococcus lactis
ethylendiaminetetraacetate 1 mM, 95% inhibition Lactococcus lactis
p-hydroxymercuribenzoate 1 mM, 40% inhibition Lactococcus lactis
phosphoramidon 1 mM, 48% inhibition Lactococcus lactis
Zn2+ 1 mM, 35% inhibition Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 2-oxoisovalerate pH 6.5, 37°C Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Lactococcus lactis 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 4 * 61000, calculated, 4 * 66000, SDS-PAGE Lactococcus lactis
66000
-
 4 * 61000, calculated, 4 * 66000, SDS-PAGE Lactococcus lactis
240000
-
 gel filtration Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis Q684J7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80.7
-
 pH 6.5, 37°C Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoisocaproate 22.7% of the activity with 2-oxoisovalerate Lactococcus lactis 3-methylbutanal + CO2
-
 ?
2-oxoisovalerate
-
 Lactococcus lactis 2-methylpropanal + CO2
-
 ?
2-oxomethylvalerate 16.7% of the activity with 2-oxoisovalerate Lactococcus lactis ?
-
 ?
3-phenylpyruvate 8.8% of the activity with 2-oxoisovalerate Lactococcus lactis 2-phenylethanal + CO2
-
 ?
4-methylthio-2-oxobutanoate 7.2% of the activity with 2-oxoisovalerate Lactococcus lactis 3-methylthiopropanal + CO2
-
 ?
indole-3-pyruvate 0.1% of the activity with 2-oxoisovalerate Lactococcus lactis 2-(indol-3-yl)-ethanal + CO2
-
 ?
pyruvate 0.6% of the activity with 2-oxoisovalerate Lactococcus lactis ethanal + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 61000, calculated, 4 * 66000, SDS-PAGE Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, complete inactivation Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
 Lactococcus lactis