Any feedback?
Literature summary for 4.1.1.1 extracted from
- Pyruvate decarboxylase, the target for omeprazole in metronidazole-resistant and iron-restricted Tritrichomonas foetus (2004), Antimicrob. Agents Chemother., 48, 2185-2189.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Omeprazole | 50% inhibition at 0.016 mg/ml for enzyme from metronidazole-resistant strain or from cells grown under iron-limited conditions, little effect on enzyme from metronidazole-susceptible strain | Tritrichomonas suis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.62 | - |
pyruvate | pH 6.2, 25°C | Tritrichomonas suis | |
| 1.25 | - |
pyruvate | pH 6.2, 25°C, presence of 0.1 M NaCl | Tritrichomonas suis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Tritrichomonas suis | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Tritrichomonas suis |
| 240000 | - |
gel filtration | Tritrichomonas suis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tritrichomonas suis | - |
activity in metronidazole- or iron-restricted cells is 20-30 times higher than in metronidazole-sensitive strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tritrichomonas suis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
pH 6.2, 25°C, metronidazole-resistant strain | Tritrichomonas suis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Tritrichomonas suis | acetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Tritrichomonas suis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.75 | 6.5 | - |
Tritrichomonas suis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
