Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Pyruvamide | activator, 2 binding sites per dimer, mode of binding, a disorder-order transition of two active-site loop regions is a key event in the activation process, kinetic data, mechanism | Saccharomyces cerevisiae | |
pyruvate | substrate activation, mechanism | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
PDC complex with pyruvamide | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Saccharomyces cerevisiae | penultimate step in the alcoholic fermentation process | acetaldehyde + CO2 | - |
? | |
pyruvate | Saccharomyces cerevisiae WS34/70 | penultimate step in the alcoholic fermentation process | acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae WS34/70 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43 | 46 | pH 6, 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | 4 active sites in the tetramer, enzyme structure | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | penultimate step in the alcoholic fermentation process | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | 4 active sites in the tetramer, enzyme structure | Saccharomyces cerevisiae WS34/70 | acetaldehyde + CO2 | - |
? | |
pyruvate | penultimate step in the alcoholic fermentation process | Saccharomyces cerevisiae WS34/70 | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | enzyme structure, differences in the tetramer assembly of form A and B PDC, form A is the native PDC | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requirement | Saccharomyces cerevisiae |