Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive towards okadaic acid and EDTA | Homo sapiens | |
additional information | insensitive towards okadaic acid and EDTA | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13700 | - |
x * 13700, mass spectrometry | Sus scrofa |
13700 | - |
x * 13700, mass spectrometry | Homo sapiens |
13701 | - |
x * 13701, calculated from amino acid sequence | Sus scrofa |
13701 | - |
x * 13701, calculated from amino acid sequence | Homo sapiens |
14000 | - |
x * 14000, SDS-PAGE | Sus scrofa |
14000 | - |
x * 14000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [protein]-N-phospho-L-histidine + H2O | Sus scrofa | - |
a [protein]-L-histidine + phosphate | - |
? | |
a [protein]-N-phospho-L-histidine + H2O | Homo sapiens | - |
a [protein]-L-histidine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NRX4 | - |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration | Sus scrofa |
DEAE-cellulose column chromatography, Sephadex G-200 gel filtration, Mono-Q column chromatography, and Superose 12 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Homo sapiens | - |
liver | - |
Sus scrofa | - |
skeletal muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0028 | - |
crude enzyme, at pH 7.5 and 30°C | Sus scrofa |
2.72 | - |
after 971fold purification, at pH 7.5 and 30°C | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [protein]-N-phospho-L-histidine + H2O | - |
Sus scrofa | a [protein]-L-histidine + phosphate | - |
? | |
a [protein]-N-phospho-L-histidine + H2O | - |
Homo sapiens | a [protein]-L-histidine + phosphate | - |
? | |
succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O | - |
Sus scrofa | succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate | - |
? | |
succinyl-L-Ala-N-phospho-L-His-L-Pro-L-Phe 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-L-Ala-L-His-L-Pro-L-Phe 4-nitroanilide + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14000, SDS-PAGE | Sus scrofa |
? | x * 14000, SDS-PAGE | Homo sapiens |
? | x * 13700, mass spectrometry | Sus scrofa |
? | x * 13700, mass spectrometry | Homo sapiens |
? | x * 13701, calculated from amino acid sequence | Sus scrofa |
? | x * 13701, calculated from amino acid sequence | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Sus scrofa |
7.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 9 | at pH 5.6, the activity is 2% of that at pH 7.5, and no activity is detected at pH 9.0 | Sus scrofa |
5.6 | 9 | at pH 5.6, the activity is 2% of that at pH 7.5, and no activity is detected at pH 9.0 | Homo sapiens |