Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
C14S | mutation of auxiliary cysteine residue present in the active site, significant resuction in kcat value | Geobacillus stearothermophilus |
C9S | mutation of active site cysteine, complete loss of activity | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-{(2S)-1-amino-5-[(2-imino-2,5-dihydro-1,3-selenazol-4-yl)amino]-1-oxopentan-2-yl}benzamide | irreversible inhibition, kinact/KI is 310 per M and min, by inducing disulfide bond formation between the two active site cysteine residues | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
4-nitrophenyl phosphate | wild-type, pH 7.6, 25°C | Geobacillus stearothermophilus | |
115 | - |
4-nitrophenyl phosphate | mutant C14S, pH 7.6, 25°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | S0F332 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YwlE | - |
Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
4-nitrophenyl phosphate | mutant C14S, pH 7.6, 25°C | Geobacillus stearothermophilus | |
1.12 | - |
4-nitrophenyl phosphate | wild-type, pH 7.6, 25°C | Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00169 | - |
4-nitrophenyl phosphate | mutant C14S, pH 7.6, 25°C | Geobacillus stearothermophilus | |
0.011 | - |
4-nitrophenyl phosphate | wild-type, pH 7.6, 25°C | Geobacillus stearothermophilus |