BRENDA - Enzyme Database
show all sequences of 3.8.1.7

Role of active site binding interactions in 4-chlorobenzoyl-coenzyme A dehalogenase catalysis

Luo, L.; Taylor, K.L.; Xiang, H.; Wei, Y.; Zhang, W.; Dunaway-Mariano, D.; Biochemistry 40, 15684-15692 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type and mutant enzymes in Escherichia coli
Pseudomonas sp.
Engineering
Amino acid exchange
Commentary
Organism
F64A
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
F64P
site-directed mutagenesis, highly decreased kcat compared to the wild-type enzyme, nearly inactive
Pseudomonas sp.
G113A
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R24K
site-directed mutagenesis, increased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R24L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R257K
site-directed mutagenesis, slightly decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
R257L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R67K
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
R67L
site-directed mutagenesis, expression in inclusion bodies
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
3'-AMP
weak inhibitor
Pseudomonas sp.
3'-dephospho-CoA
-
Pseudomonas sp.
4-Chlorobenzoate
poor inhibitor
Pseudomonas sp.
4-chlorobenzoyl-coenzyme A
competitive
Pseudomonas sp.
4-hydroxybenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
4-hydroxybenzoyl-CoA
competitive
Pseudomonas sp.
4-hydroxybenzoyl-dithio-CoA
competitive
Pseudomonas sp.
4-methylbenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
4-methylbenzoyl-CoA
competitive
Pseudomonas sp.
5'-ADP
weak inhibitor
Pseudomonas sp.
5'-AMP
weak inhibitor
Pseudomonas sp.
adenosine
weak inhibitor
Pseudomonas sp.
adenosine 3',5'-bisphosphate
weak inhibitor
Pseudomonas sp.
ATP
weak inhibitor
Pseudomonas sp.
CoA
feedback inhibition
Pseudomonas sp.
diphosphate
weak inhibitor
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas sp.
0.0037
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.0052
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25°C
Pseudomonas sp.
0.0058
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.0069
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.014
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.02
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.021
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.024
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.033
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.041
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.07
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.07
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25°C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
0.11
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25°C
Pseudomonas sp.
0.31
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.44
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
2
-
4-chlorobenzoyl-pantetheine
above, wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-chlorobenzoyl-CoA + H2O
Pseudomonas sp.
degradation in soil, pathway overview
4-hydroxybenzoyl-CoA + chloride
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Reaction
Reaction
Commentary
Organism
4-chlorobenzoyl-CoA + H2O = 4-hydroxybenzoyl CoA + chloride
specific for dehalogenation at the 4-position, can dehalogenate substrates bearing fluorine, chlorine, bromine and iodine in the 4-position, multistep mechanism, attack of Asp145 on the C(4) of the substrates' benzoyl ring to form a Meisenheimer intermediate, followed by expulsion of chloride ion to form an arylated enzyme intermediate, and ester hydrolysis to form 4-hydroxybenzoyl-CoA, active site structure
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-bromophenacyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxyphenacyl-CoA + bromide
-
-
-
?
4-chlorobenzoyl-3'-dephospho-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-3'-dephospho-CoA + chloride
-
-
-
?
4-chlorobenzoyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
4-chlorobenzoyl-CoA + H2O
degradation in soil, pathway overview
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
4-chlorobenzoyl-dithio-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-dithio-CoA + chloride
-
-
-
?
4-chlorobenzoyl-epsilon-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-epsilon-CoA + chloride
-
-
-
?
4-chlorobenzoyl-pantetheine + H2O
very low activity
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine + chloride
-
-
-
?
4-chlorobenzoyl-pantetheine phosphate + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine phosphate + chloride
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00001
-
4-chlorobenzoyl-CoA
below, mutant F64P, pH 7.5, 25°C
Pseudomonas sp.
0.001
-
4-chlorobenzoyl-pantetheine
about, wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.002
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25°C
Pseudomonas sp.
0.003
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.005
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.007
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.03
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.03
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25°C
Pseudomonas sp.
0.051
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.056
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25°C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
0.14
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.33
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.38
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.45
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.55
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.6
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.95
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Pseudomonas sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0017
-
4-hydroxybenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.0024
-
4-hydroxybenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.0025
-
4-hydroxybenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.0031
-
4-methylbenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.0042
-
4-methylbenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.0062
-
4-hydroxybenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.011
-
4-methylbenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.0165
-
4-hydroxybenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.019
-
4-hydroxybenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.022
-
4-methylbenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.04
-
4-hydroxybenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.044
-
4-methylbenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.046
-
4-methylbenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.05
0.1
4-hydroxybenzoyl-CoA
mutant G114A, pH 7.5, 25°C
Pseudomonas sp.
0.051
-
4-methylbenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.055
-
4-chlorobenzoyl-coenzyme A
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.069
-
4-hydroxybenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.072
-
4-methylbenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.09
-
CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.14
-
CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.25
-
CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.52
-
CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.66
-
CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.77
-
3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
2.4
-
CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
4.2
-
5'-ADP
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
21
-
4-Chlorobenzoate
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutant enzymes in Escherichia coli
Pseudomonas sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F64A
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
F64P
site-directed mutagenesis, highly decreased kcat compared to the wild-type enzyme, nearly inactive
Pseudomonas sp.
G113A
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R24K
site-directed mutagenesis, increased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R24L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R257K
site-directed mutagenesis, slightly decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
R257L
site-directed mutagenesis, decreased kcat and increased Km compared to the wild-type enzyme
Pseudomonas sp.
R67K
site-directed mutagenesis, decreased kcat and slightly increased Km compared to the wild-type enzyme
Pseudomonas sp.
R67L
site-directed mutagenesis, expression in inclusion bodies
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3'-AMP
weak inhibitor
Pseudomonas sp.
3'-dephospho-CoA
-
Pseudomonas sp.
4-Chlorobenzoate
poor inhibitor
Pseudomonas sp.
4-chlorobenzoyl-coenzyme A
competitive
Pseudomonas sp.
4-hydroxybenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
4-hydroxybenzoyl-CoA
competitive
Pseudomonas sp.
4-hydroxybenzoyl-dithio-CoA
competitive
Pseudomonas sp.
4-methylbenzoyl-3'-dephospho-CoA
competitive
Pseudomonas sp.
4-methylbenzoyl-CoA
competitive
Pseudomonas sp.
5'-ADP
weak inhibitor
Pseudomonas sp.
5'-AMP
weak inhibitor
Pseudomonas sp.
adenosine
weak inhibitor
Pseudomonas sp.
adenosine 3',5'-bisphosphate
weak inhibitor
Pseudomonas sp.
ATP
weak inhibitor
Pseudomonas sp.
CoA
feedback inhibition
Pseudomonas sp.
diphosphate
weak inhibitor
Pseudomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0017
-
4-hydroxybenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.0024
-
4-hydroxybenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.0025
-
4-hydroxybenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.0031
-
4-methylbenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.0042
-
4-methylbenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.0062
-
4-hydroxybenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.011
-
4-methylbenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.0165
-
4-hydroxybenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.019
-
4-hydroxybenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.022
-
4-methylbenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.04
-
4-hydroxybenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.044
-
4-methylbenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.046
-
4-methylbenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.05
0.1
4-hydroxybenzoyl-CoA
mutant G114A, pH 7.5, 25°C
Pseudomonas sp.
0.051
-
4-methylbenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.055
-
4-chlorobenzoyl-coenzyme A
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.069
-
4-hydroxybenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.072
-
4-methylbenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.09
-
CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.14
-
CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.25
-
CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.52
-
CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.66
-
CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.77
-
3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
2.4
-
CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
4.2
-
5'-ADP
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
21
-
4-Chlorobenzoate
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas sp.
0.0037
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.0052
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25°C
Pseudomonas sp.
0.0058
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.0069
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.014
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.02
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.021
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
0.024
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.033
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.041
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.07
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.07
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25°C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
0.11
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25°C
Pseudomonas sp.
0.31
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.44
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
2
-
4-chlorobenzoyl-pantetheine
above, wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-chlorobenzoyl-CoA + H2O
Pseudomonas sp.
degradation in soil, pathway overview
4-hydroxybenzoyl-CoA + chloride
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-bromophenacyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxyphenacyl-CoA + bromide
-
-
-
?
4-chlorobenzoyl-3'-dephospho-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-3'-dephospho-CoA + chloride
-
-
-
?
4-chlorobenzoyl-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
4-chlorobenzoyl-CoA + H2O
degradation in soil, pathway overview
654589
Pseudomonas sp.
4-hydroxybenzoyl-CoA + chloride
-
-
-
?
4-chlorobenzoyl-dithio-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-dithio-CoA + chloride
-
-
-
?
4-chlorobenzoyl-epsilon-CoA + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-epsilon-CoA + chloride
-
-
-
?
4-chlorobenzoyl-pantetheine + H2O
very low activity
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine + chloride
-
-
-
?
4-chlorobenzoyl-pantetheine phosphate + H2O
-
654589
Pseudomonas sp.
4-hydroxybenzoyl-pantetheine phosphate + chloride
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00001
-
4-chlorobenzoyl-CoA
below, mutant F64P, pH 7.5, 25°C
Pseudomonas sp.
0.001
-
4-chlorobenzoyl-pantetheine
about, wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.002
-
4-chlorobenzoyl-CoA
mutant G114A, pH 7.5, 25°C
Pseudomonas sp.
0.003
-
4-chlorobenzoyl-dithio-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.005
-
4-chlorobenzoyl-CoA
mutant G113A, pH 7.5, 25°C
Pseudomonas sp.
0.007
-
4-chlorobenzoyl-epsilon-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.03
-
4-bromophenacyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.03
-
4-chlorobenzoyl-CoA
mutant F64A, pH 7.5, 25°C
Pseudomonas sp.
0.051
-
4-chlorobenzoyl-pantetheine phosphate
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.056
-
4-chlorobenzoyl-pantetheine
wild-type enzyme, pH 7.5, 25°C, in presence of 1 mM 5'-ADP
Pseudomonas sp.
0.14
-
4-chlorobenzoyl-CoA
mutant R257L, pH 7.5, 25°C
Pseudomonas sp.
0.33
-
4-chlorobenzoyl-CoA
mutant R24L, pH 7.5, 25°C
Pseudomonas sp.
0.38
-
4-chlorobenzoyl-CoA
mutant R67K, pH 7.5, 25°C
Pseudomonas sp.
0.45
-
4-chlorobenzoyl-3'-dephospho-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.55
-
4-chlorobenzoyl-CoA
mutant R257K, pH 7.5, 25°C
Pseudomonas sp.
0.6
-
4-chlorobenzoyl-CoA
wild-type enzyme, pH 7.5, 25°C
Pseudomonas sp.
0.95
-
4-chlorobenzoyl-CoA
mutant R24K, pH 7.5, 25°C
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Pseudomonas sp.
Other publictions for EC 3.8.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720401
Plotnikova
Degradation of 4-chlorobipheny ...
Rhodococcus ruber, Rhodococcus ruber P25
Microbiology
81
143-153
2012
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719717
Wang
A novel hydrolytic dehalogenas ...
Pseudomonas sp., Pseudomonas sp. CTN-3
J. Bacteriol.
192
2737-2745
2010
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7
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7
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2
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2
6
1
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3
1
1
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1
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1
685426
Zhou
Structure and denaturation of ...
Arthrobacter sp.
Biodegradation
19
65-75
2008
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1
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697974
Chae
Identification of genes coding ...
consortium cosmid clone pGZ1
FEMS Microbiol. Lett.
281
203-209
2008
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667699
Wu
Contributions of long-range el ...
Pseudomonas sp.
Biochemistry
45
102-112
2006
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668613
Xu
Electrostatic influence of act ...
Pseudomonas sp.
FEBS Lett.
579
4249-4253
2005
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670022
Dong
-
Raman evidence for product bin ...
Pseudomonas sp.
J. Raman Spectros.
36
320-325
2005
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655227
Xu
-
A QM/MM study of a nucleophili ...
Bacteria
Chem. Commun. (Camb.)
2004
892-893
2004
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661288
Zhou
The purification and character ...
Arthrobacter sp., Arthrobacter sp. TM-1
Biodegradation
15
97-109
2004
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1
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5
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2
1
1
1
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1
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1
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669044
Xu
QM/MM studies of the enzyme-ca ...
Pseudomonas sp.
J. Am. Chem. Soc.
126
13649-13658
2004
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1
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654718
Dong
The strength of dehalogenase-s ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
42
9482-9490
2003
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1
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14
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5
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2
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10
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1
1
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7
1
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14
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7
1
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654585
Zhang
Histidine 90 function in 4-chl ...
Pseudomonas sp.
Biochemistry
40
13474-13482
2001
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1
1
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1
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5
1
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5
1
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654589
Luo
Role of active site binding in ...
Pseudomonas sp.
Biochemistry
40
15684-15692
2001
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1
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9
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16
17
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1
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1
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8
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1
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17
1
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27
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1
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9
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16
27
17
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1
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8
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1
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17
1
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657187
Lau
The active site dynamics of 4- ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Proc. Natl. Acad. Sci. USA
98
9527-9532
2001
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33080
Taylor
Investigation of substrate act ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
36
1349-1361
1997
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7
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11
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33081
Benning
Structure of 4-chlorobenzoyl c ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
35
8103-8109
1996
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1
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3
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33085
Yang
Identification of active site ...
Pseudomonas sp.
Biochemistry
35
10879-10885
1996
-
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3
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2
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1
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33082
Löffler
Dehalogenation of 4-chlorobenz ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biodegradation
6
203-212
1995
-
-
-
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1
9
1
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2
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12
-
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1
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1
1
8
3
1
1
3
1
1
1
5
-
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1
-
9
-
1
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2
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1
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1
1
8
3
1
1
3
1
1
1
5
-
-
-
-
-
-
-
33086
Crooks
-
Exploration of possible mechan ...
Arthrobacter sp., Arthrobacter sp. 4-CB1
J. Am. Chem. Soc.
117
10791-10798
1995
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4
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781
Dunaway-Mariano
On the origins and functions o ...
Arthrobacter sp. 4-CB1, Arthrobacter sp., Pseudomonas sp., Pseudomonas sp. CBS-3
Biodegradation
5
259-276
1994
-
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1
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3
8
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4
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16
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20
2
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5
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20
2
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5
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33077
Crooks
Purification and characterizat ...
Arthrobacter sp. 4-CB1, Arthrobacter sp.
Biochemistry
33
11645-11649
1994
-
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5
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8
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1
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1
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10
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4
1
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2
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5
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1
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1
-
10
-
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4
1
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2
-
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-
33083
Yang
Evidence for nucleophilic cata ...
Pseudomonas sp.
Biochemistry
33
8527-8531
1994
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33078
Liang
Specificity of 4-chlorobenzoyl ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
32
12245-12250
1993
-
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1
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3
8
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11
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13
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7
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1
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13
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7
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780
Chang
Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
31
5605-5610
1992
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1
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3
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12
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