Activating Compound | Comment | Organism | Structure |
---|---|---|---|
imidazole | imidazole buffers promote the enzyme activity | Alcanivorax dieselolei |
Application | Comment | Organism |
---|---|---|
environmental protection | enzyme DadB and its host, Alcanivorax dieselolei strain B-5, are of potential use for biocatalysis and bioremediation applications | Alcanivorax dieselolei |
synthesis | enzyme DadB and its host, Alcanivorax dieselolei strain B-5, are of potential use for biocatalysis and bioremediation applications | Alcanivorax dieselolei |
Cloned (Comment) | Organism |
---|---|
gene dadB, phylogenetic analysis | Alcanivorax dieselolei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(morpholin-4-yl)propane-1-sulfonic acid | phosphate and MOPS buffers repress the enzyme activity | Alcanivorax dieselolei | |
additional information | no activity in glycine buffers | Alcanivorax dieselolei | |
phosphate | phosphate and MOPS buffers repress the enzyme activity | Alcanivorax dieselolei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Alcanivorax dieselolei | |
0.82 | - |
1,3-dibromopropane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
4.88 | - |
1-Chlorobutane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
8.47 | - |
4-bromobutyronitrile | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
8.7 | - |
1,2-Dibromoethane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
11.33 | - |
3-chloro-2-methylprop-1-ene | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
15.06 | - |
2,3-dichloroprop-1-ene | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-haloalkane + H2O | Alcanivorax dieselolei | - |
a primary alcohol + halide | - |
? | |
additional information | Alcanivorax dieselolei | enzyme DadB possesses broad substrate range and has the highest overall activity among the identified haloalkanes dehalogenases. The enzyme prefers brominated substrates, chlorinated alkenes, and the C2-C3 substrates, including the persistent pollutants of 1,2-dichloroethane, 1,2-dichloropropane and 1,2,3-trichloropropane, but displays no detectable activity toward long-chain haloalkanes such as 1-chlorohexadecane and 1-chlorooctadecane | ? | - |
? | |
additional information | Alcanivorax dieselolei B-5 | enzyme DadB possesses broad substrate range and has the highest overall activity among the identified haloalkanes dehalogenases. The enzyme prefers brominated substrates, chlorinated alkenes, and the C2-C3 substrates, including the persistent pollutants of 1,2-dichloroethane, 1,2-dichloropropane and 1,2,3-trichloropropane, but displays no detectable activity toward long-chain haloalkanes such as 1-chlorohexadecane and 1-chlorooctadecane | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcanivorax dieselolei | K0CAI2 | gene dadB | - |
Alcanivorax dieselolei B-5 | K0CAI2 | gene dadB | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chlorotetradecane | Alcanivorax dieselolei |
0.018 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chlorododecane | Alcanivorax dieselolei |
0.024 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chloro-2-methylpropane | Alcanivorax dieselolei |
0.06 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate dichloromethane | Alcanivorax dieselolei |
0.084 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chlorodecane | Alcanivorax dieselolei |
0.28 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate (bromomethyl)cyclohexane | Alcanivorax dieselolei |
0.31 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2-dichloropropane | Alcanivorax dieselolei |
0.34 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2,3-trichloropropane | Alcanivorax dieselolei |
0.702 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2-dichloroethane | Alcanivorax dieselolei |
0.76 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chlorohexane | Alcanivorax dieselolei |
0.862 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-bromohexane | Alcanivorax dieselolei |
0.864 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,5-dichloropentane | Alcanivorax dieselolei |
0.92 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-iodohexane | Alcanivorax dieselolei |
1.11 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chlorobutane | Alcanivorax dieselolei |
1.48 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate bromocyclohexane | Alcanivorax dieselolei |
2.4 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,3-diiodopropane | Alcanivorax dieselolei |
3.04 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-iodobutane | Alcanivorax dieselolei |
3.54 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate chlorocyclopentane | Alcanivorax dieselolei |
3.63 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chloro-2-(2-chloroethoxy)ethane | Alcanivorax dieselolei |
3.88 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-iodopropane | Alcanivorax dieselolei |
4.51 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-bromobutane | Alcanivorax dieselolei |
5.33 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,3-dichloropropane | Alcanivorax dieselolei |
7.48 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2-dibromopropane | Alcanivorax dieselolei |
8.14 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2-dibromo-3-chloropropane | Alcanivorax dieselolei |
8.3 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 2-bromo-1-chloropropane | Alcanivorax dieselolei |
9.92 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-bromo-3-chloropropane | Alcanivorax dieselolei |
10.3 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,3-dibromopropane | Alcanivorax dieselolei |
11.53 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-bromo-2-chloroethane | Alcanivorax dieselolei |
12.66 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1-chlorooctane | Alcanivorax dieselolei |
13.54 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,3-dichloropropene | Alcanivorax dieselolei |
14.05 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 2,3-dichloroprop-1-ene | Alcanivorax dieselolei |
14.16 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2-dibromoethane | Alcanivorax dieselolei |
14.44 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 4-bromobutyronitrile | Alcanivorax dieselolei |
15.13 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2,3-tribromopropane | Alcanivorax dieselolei |
24.05 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 3-chloro-2-methylprop-1-ene | Alcanivorax dieselolei |
26.48 | - |
pH 8.6, 37°C, purified recombinant enzyme, substrate 1,2,3-trichloropropene | Alcanivorax dieselolei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(bromomethyl)cyclohexane + H2O | - |
Alcanivorax dieselolei | cyclohexylmethanol + bromide | - |
? | |
1,2,3-tribromopropane + H2O | - |
Alcanivorax dieselolei | 2,3-dibromopropanol + bromide | - |
? | |
1,2,3-trichloropropane + H2O | - |
Alcanivorax dieselolei | 2,3-dichloropropanol + chloride | - |
? | |
1,2,3-trichloropropene + H2O | - |
Alcanivorax dieselolei | 2,3-dichloropropenol + chloride | - |
? | |
1,2-dibromo-3-chloropropane + H2O | - |
Alcanivorax dieselolei | 2-bromo-3-chloropropanol + bromide | - |
? | |
1,2-dibromoethane + H2O | - |
Alcanivorax dieselolei | 2-bromoethanol + bromide | - |
? | |
1,2-dibromoethane + H2O | - |
Alcanivorax dieselolei B-5 | 2-bromoethanol + bromide | - |
? | |
1,2-dibromopropane + H2O | - |
Alcanivorax dieselolei | 2-bromopropanol + bromide | - |
? | |
1,2-dichloroethane + H2O | - |
Alcanivorax dieselolei | 2-chloroethanol + chloride | - |
? | |
1,2-dichloroethane + H2O | - |
Alcanivorax dieselolei B-5 | 2-chloroethanol + chloride | - |
? | |
1,2-dichloropropane + H2O | - |
Alcanivorax dieselolei | 2-chloropropanol + chloride | - |
? | |
1,3-dibromopropane + H2O | - |
Alcanivorax dieselolei | 3-bromopropanol + bromide | - |
? | |
1,3-dichloropropane + H2O | - |
Alcanivorax dieselolei | 3-chloropropanol + chloride | - |
? | |
1,3-dichloropropene + H2O | - |
Alcanivorax dieselolei | 3-chloropropenol + chloride | - |
? | |
1,3-diiodopropane + H2O | - |
Alcanivorax dieselolei | 3-iodopropanol + iodide | - |
? | |
1,5-dichloropentane + H2O | - |
Alcanivorax dieselolei | 5-chloropentanol + chloride | - |
? | |
1-bromo-2-chloroethane + H2O | - |
Alcanivorax dieselolei | 2-chloroethanol + bromide | - |
? | |
1-bromo-3-chloropropane + H2O | - |
Alcanivorax dieselolei | 3-chloropropanol + bromide | - |
? | |
1-bromobutane + H2O | - |
Alcanivorax dieselolei | 1-butanol + bromide | - |
? | |
1-bromohexane + H2O | - |
Alcanivorax dieselolei | 1-hexanol + bromide | - |
? | |
1-chloro-2-(2-chloroethoxy)ethane + H2O | - |
Alcanivorax dieselolei | 2-(2-chloroethoxy)ethanol + chloride | - |
? | |
1-chloro-2-methylpropane + H2O | - |
Alcanivorax dieselolei | 2-methylpropanol + chloride | - |
? | |
1-chlorobutane + H2O | - |
Alcanivorax dieselolei | 1-butanol + chloride | - |
? | |
1-chlorodecane + H2O | - |
Alcanivorax dieselolei | decanol + chloride | - |
? | |
1-chlorododecane + H2O | - |
Alcanivorax dieselolei | dodecanol + chloride | - |
? | |
1-chlorohexane + H2O | - |
Alcanivorax dieselolei | 1-hexanol + chloride | - |
? | |
1-chlorooctane + H2O | - |
Alcanivorax dieselolei | octanol + chloride | - |
? | |
1-chlorotetradecane + H2O | - |
Alcanivorax dieselolei | tetradecanol + chloride | - |
? | |
1-haloalkane + H2O | - |
Alcanivorax dieselolei | a primary alcohol + halide | - |
? | |
1-iodobutane + H2O | - |
Alcanivorax dieselolei | butanol + iodide | - |
? | |
1-iodohexane + H2O | - |
Alcanivorax dieselolei | hexanol + iodide | - |
? | |
1-iodopropane + H2O | - |
Alcanivorax dieselolei | propanol + iodide | - |
? | |
1-iodopropane + H2O | - |
Alcanivorax dieselolei B-5 | propanol + iodide | - |
? | |
2,3-dichloroprop-1-ene + H2O | - |
Alcanivorax dieselolei | ? | - |
? | |
3-chloro-2-methylprop-1-ene + H2O | - |
Alcanivorax dieselolei | ? | - |
? | |
4-bromobutyronitrile + H2O | - |
Alcanivorax dieselolei | 4-hydroxybutyronitrile + bromide | - |
? | |
bromocyclohexane + H2O | - |
Alcanivorax dieselolei | cyclohexanol + bromide | - |
? | |
chlorocyclopentane + H2O | - |
Alcanivorax dieselolei | cyclopentanol + chloride | - |
? | |
dichloromethane + H2O | - |
Alcanivorax dieselolei | ? | - |
? | |
additional information | enzyme DadB possesses broad substrate range and has the highest overall activity among the identified haloalkanes dehalogenases. The enzyme prefers brominated substrates, chlorinated alkenes, and the C2-C3 substrates, including the persistent pollutants of 1,2-dichloroethane, 1,2-dichloropropane and 1,2,3-trichloropropane, but displays no detectable activity toward long-chain haloalkanes such as 1-chlorohexadecane and 1-chlorooctadecane | Alcanivorax dieselolei | ? | - |
? | |
additional information | the enzyme DadB prefers brominated substrates over than their chlorinated and iodinated counterparts, its activities against brominated C1-C4 substrates are generally 20 times higher than the corresponding chlorinated substrates. In the case of halogenated butane, the activities are 1-bromobutane > 1-iodobutane > 1-chlorobutane. For 1,3-dihalogenated propane, the activities are 1,3-dibromopropane > 1-chloro-3-bromopropane > 1,3-dichloropropane > 1,3-diiodopropane. The activity of enzyme DadB against chlorinated alkenes, such as 2,3-dichloroprop-1-ene, 3-chloro-2-methylprop-1-ene, 1,3-dichloropropene, and 1,2,3-trichloropropene, is higher than the corresponding chlorinated alkanes. Substrate specificity, overview. No activity with 2-iodobutane, chlorocyclohexane, 1-chlorohexadecane, 1-bromohexadecane, 1-chlorooctadecane, trichloromethane, 1-chloro-3-nitrobenzene, 4-bromodiphenyl ether, decabromodiphenyl, and trichloroacetic acid | Alcanivorax dieselolei | ? | - |
? | |
additional information | enzyme DadB possesses broad substrate range and has the highest overall activity among the identified haloalkanes dehalogenases. The enzyme prefers brominated substrates, chlorinated alkenes, and the C2-C3 substrates, including the persistent pollutants of 1,2-dichloroethane, 1,2-dichloropropane and 1,2,3-trichloropropane, but displays no detectable activity toward long-chain haloalkanes such as 1-chlorohexadecane and 1-chlorooctadecane | Alcanivorax dieselolei B-5 | ? | - |
? | |
additional information | the enzyme DadB prefers brominated substrates over than their chlorinated and iodinated counterparts, its activities against brominated C1-C4 substrates are generally 20 times higher than the corresponding chlorinated substrates. In the case of halogenated butane, the activities are 1-bromobutane > 1-iodobutane > 1-chlorobutane. For 1,3-dihalogenated propane, the activities are 1,3-dibromopropane > 1-chloro-3-bromopropane > 1,3-dichloropropane > 1,3-diiodopropane. The activity of enzyme DadB against chlorinated alkenes, such as 2,3-dichloroprop-1-ene, 3-chloro-2-methylprop-1-ene, 1,3-dichloropropene, and 1,2,3-trichloropropene, is higher than the corresponding chlorinated alkanes. Substrate specificity, overview. No activity with 2-iodobutane, chlorocyclohexane, 1-chlorohexadecane, 1-bromohexadecane, 1-chlorooctadecane, trichloromethane, 1-chloro-3-nitrobenzene, 4-bromodiphenyl ether, decabromodiphenyl, and trichloroacetic acid | Alcanivorax dieselolei B-5 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | DadB aggregates fast in the buffers with pH below pH 7.0, while keeps stable in monomer form when pH is above pH 7.5 | Alcanivorax dieselolei |
Synonyms | Comment | Organism |
---|---|---|
DadB | - |
Alcanivorax dieselolei |
HLD | - |
Alcanivorax dieselolei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Alcanivorax dieselolei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | the enzyme activity increases gradually from 20°C to 50°C and decreases above 50°C, with the temperature optimum being 50°C, inactive at 60°C | Alcanivorax dieselolei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.17 | - |
1-Chlorobutane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
13.39 | - |
1,3-dibromopropane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
17.95 | - |
1,2-Dibromoethane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
20.06 | - |
2,3-dichloroprop-1-ene | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
30.8 | - |
4-bromobutyronitrile | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
34.26 | - |
3-chloro-2-methylprop-1-ene | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | peak 1, first optimum | Alcanivorax dieselolei |
8 | 9 | peak 2, second optimum | Alcanivorax dieselolei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range | Alcanivorax dieselolei |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme DadB likely belongs to the HLD-II subfamily, closely related with LinB and DmbA, while DadA appears to be relatively independent from the HLD-II subfamily. This pentad includes the nucleophile residue D108, the basic residue H271, the acid catalytic residue E132, and the halide-binding residues N37 and W109. DadB possesses a large main tunnel opening, but it prefers small substrates | Alcanivorax dieselolei |
additional information | DadB has an open active cavity with a large access tunnel, which is supposed important for larger molecules as opposed to C2-C3 substrates, homology modeling, residue I247 plays an important role in substrate selection. Homology modeling and structural analysis, overview. Enzyme DadB is composed of a core domain and a cap domain, identical catalytic pentad of HLD-II subfamily | Alcanivorax dieselolei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
1-Chlorobutane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
1.33 | - |
2,3-dichloroprop-1-ene | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
2.06 | - |
1,2-Dibromoethane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
3.02 | - |
3-chloro-2-methylprop-1-ene | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
3.64 | - |
4-bromobutyronitrile | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei | |
16.43 | - |
1,3-dibromopropane | pH 8.6, 37°C, recombinant enzyme | Alcanivorax dieselolei |