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Literature summary for 3.8.1.5 extracted from

  • Ito, M.; Prokop, Z.; Klvana, M.; Otsubo, Y.; Tsuda, M.; Damborsky, J.; Nagata, Y.
    Degradation of beta-hexachlorocyclohexane by haloalkane dehalogenase LinB from gamma-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI1205 (2007), Arch. Microbiol., 188, 313-325.
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
using the plasmids pKS13, pUC18, pUC19 and pAQN, the plasmid pAQN is used for epression of the protein in Escherichia coli BL21 cells Sphingobium indicum
using the plasmids pKS13, pUC18, pUC19 and pAQNM, the plasmid pAQNM is used for expression of the protein in Escherichia coli BL21 cells Sphingobium sp.

Protein Variants

Protein Variants Comment Organism
A247H mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. Sphingobium indicum
H247A mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. Sphingobium sp.
I134A mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. Sphingobium indicum
I134V/A247H mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. Sphingobium indicum
V134I mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. Sphingobium sp.
V134I/H247A mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. Sphingobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3,4,5,6-pentachlorocyclohexanol + H2O Sphingobium sp.
-
 2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
-
 ?
2,3,4,5,6-pentachlorocyclohexanol + H2O Sphingobium sp. MI1205
-
 2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O Sphingobium indicum
-
 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O Sphingobium sp. two-step conversion of beta-HCH to TCDL 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O Sphingobium sp. MI1205 two-step conversion of beta-HCH to TCDL 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O Sphingobium indicum UT26
-
 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium indicum A8CFB7
-
-
Sphingobium indicum UT26 A8CFB7
-
-
Sphingobium sp. A4PEU6
-
-
Sphingobium sp. MI1205 A4PEU6
-
-

Purification (Commentary)

Purification (Comment) Organism
using TALON metal affinity resin Sphingobium indicum
using TALON metal affinity resin Sphingobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific constant of LinB, mutant A247H, step HCH to PCHL, 0.0271 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, mutant A247H, step PCHL to TCDL, not determined Sphingobium indicum
additional information
-
 specific constant of LinB, mutant H247A, step HCH to PCHL, 0.210 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, mutant H247A, step PCHL to TCDL, 0.240 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, mutant I134V, step HCH to PCHL, 0.0969 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, mutant I134V, step PCHL to TCDL, 0.0099 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, mutant I134V/A247H, step HCH to PCHL, 0.0243 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, mutant I134V/A247H, step PCHL to TCDL, 0.0183 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, mutant V134I, step HCH to PCHL, 0.124 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, mutant V134I, step PCHL to TCDL, 0.080 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, mutant V134I/H247A, step HCH to PCHL, 0.104 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, mutant V134I/H247A, step PCHL to TCDL, 0.027 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, wild-type, step HCH to PCHL, 0.0271 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, wild-type, step HCH to PCHL, 0.205 mM-1 s-1 Sphingobium sp.
additional information
-
 specific constant of LinB, wild-type, step PCHL to TCDL, 0.0036 mM-1 s-1 Sphingobium indicum
additional information
-
 specific constant of LinB, wild-type, step PCHL to TCDL, 0.716 mM-1 s-1 Sphingobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,4,5,6-pentachlorocyclohexanol + H2O
-
 Sphingobium sp. 2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
-
 ?
2,3,4,5,6-pentachlorocyclohexanol + H2O
-
 Sphingobium sp. MI1205 2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O two-step conversion of beta-HCH to TCDL Sphingobium sp. 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O two-step conversion of beta-HCH to TCDL Sphingobium sp. MI1205 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum UT26 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?

Synonyms

Synonyms Comment Organism
haloalkane dehalogenase
-
 Sphingobium indicum
haloalkane dehalogenase
-
 Sphingobium sp.
LinB
-
 Sphingobium indicum
LinB
-
 Sphingobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 enzymatic assay Sphingobium indicum
30
-
 enzymatic assay Sphingobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 enzymatic assay Sphingobium indicum
7.5
-
 enzymatic assay Sphingobium sp.