Application | Comment | Organism |
---|---|---|
environmental protection | haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants | Bradyrhizobium japonicum |
Cloned (Comment) | Organism |
---|---|
the plasmid pYBJA2 is used for expression of the protein in Escherichia coli BL21 cells, the DbjA construct codes for residues 1-310 of DbjA with its C-terminus fused to a His6-tag | Bradyrhizobium japonicum |
Crystallization (Comment) | Organism |
---|---|
the crystal diffracts to 1.75 A resolution | Bradyrhizobium japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-haloalkane + H2O | Bradyrhizobium japonicum | - |
a primary alcohol + halide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni Chelating Sepharose Fast Flow column | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-haloalkane + H2O | - |
Bradyrhizobium japonicum | a primary alcohol + halide | - |
? | |
additional information | DjA has a high catalytic activity for beta-methylated haloalkanes | Bradyrhizobium japonicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DbjA | - |
Bradyrhizobium japonicum |
haloalkane dehalogenase | - |
Bradyrhizobium japonicum |