Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Sphingomonas paucimobilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31700 | - |
x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry | Sphingomonas paucimobilis |
33800 | - |
x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry | Sphingomonas paucimobilis |
34800 | - |
x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | P51698 | expression in Pichia pastoris | - |
Sphingomonas paucimobilis UT26 | P51698 | expression in Pichia pastoris | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme contains a 2.8 kDa high mannose core, glycosylation has no effect on activity, protein folding or thermal stability | Sphingomonas paucimobilis |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Pichia pastoris | Sphingomonas paucimobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.86 | - |
substrate 1-chlorobutane, 37°C, pH 8.6 | Sphingomonas paucimobilis |
15.6 | - |
substrate 1,2-dibromoethane, 37°C, pH 8.6 | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dibromoethane + H2O | - |
Sphingomonas paucimobilis | 2-bromoethanol + bromide | - |
? | |
1,2-dibromoethane + H2O | - |
Sphingomonas paucimobilis UT26 | 2-bromoethanol + bromide | - |
? | |
1-chlorobutane + H2O | - |
Sphingomonas paucimobilis | butanol + chloride | - |
r | |
1-chlorobutane + H2O | - |
Sphingomonas paucimobilis UT26 | butanol + chloride | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 31700, expression in Escherichia coli, x * 34800, expression in Pichia pastoris, SDS-PAGE. x * 33800, His-tagged enzyme expressed in Escherichia coli, mass spectrometry | Sphingomonas paucimobilis |
Synonyms | Comment | Organism |
---|---|---|
LinB | - |
Sphingomonas paucimobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41.7 | - |
melting temperature, enzyme expressed in Pichia pastoris | Sphingomonas paucimobilis |
41.8 | - |
melting tempreature, enzyme expressed in Escherichia coli | Sphingomonas paucimobilis |