Application | Comment | Organism |
---|---|---|
biotechnology | hydrolysis of haloalkanes by lyophilized cells in solid-gas biofilter, analysis of reaction with 1-chlorobutane. Activity and stability of cells depends on the quantitiy of HCl produced. Triethylamine is used as a volatile buffer that controls the local pH-value and the dehalogenization state of enzyme. Cells broken by lysozyme are more stable than intact cells. Initial reaction rate of 4.5 micromol per min and mg of cell is observed | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
1-Chlorobutane | lyophilized cells in soli-gas biofilter | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
lyophilized cells | - |
Rhodococcus erythropolis NCIMB 13064 | - |
lyophilized cells | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-haloalkane + H2O = a primary alcohol + halide | activation energy of reaction 59.5 kJ/mol | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chlorobutane + H2O | - |
Rhodococcus erythropolis | butanol + chloride | - |
r | |
1-chlorobutane + H2O | - |
Rhodococcus erythropolis NCIMB 13064 | butanol + chloride | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at, compromise between stability and activity | Rhodococcus erythropolis |