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Literature summary for 3.8.1.5 extracted from

  • Erable, B.; Maugard, T.; Goubet, I.; Lamare, S.; Legoy, M.D.
    Biotransformation of halogenated compounds by lyophilized cells of Rhodococcus erythropolis in a continuous solid-gas biofilter (2005), Process Biochem., 40, 45-51.
No PubMed abstract available

Application

Application Comment Organism
biotechnology hydrolysis of haloalkanes by lyophilized cells in solid-gas biofilter, analysis of reaction with 1-chlorobutane. Activity and stability of cells depends on the quantitiy of HCl produced. Triethylamine is used as a volatile buffer that controls the local pH-value and the dehalogenization state of enzyme. Cells broken by lysozyme are more stable than intact cells. Initial reaction rate of 4.5 micromol per min and mg of cell is observed Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
1-Chlorobutane lyophilized cells in soli-gas biofilter Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
lyophilized cells
-
Rhodococcus erythropolis NCIMB 13064
-
lyophilized cells
-

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide activation energy of reaction 59.5 kJ/mol Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chlorobutane + H2O
-
Rhodococcus erythropolis butanol + chloride
-
r
1-chlorobutane + H2O
-
Rhodococcus erythropolis NCIMB 13064 butanol + chloride
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at, compromise between stability and activity Rhodococcus erythropolis