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Literature summary for 3.8.1.5 extracted from

  • Pavlova, M.; Klva?a, M.; Jesenska, A.; Prokop, Z.; Kone?na, H.; Sato, T.; Tsuda, M.; Nagata, Y.; Damborsky, J.
    The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: Reaction mechanism and molecular evolution (2006), J. Struct. Biol., 157, 384-392.
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
the coding sequence is amplified in the pAL-781-dhmA vector, the vector pUC18-dhmAHis is propagated in Escherichia coli JM110, the dhmAHis gene is ligated into the vector pK4RP for expression in Rhodococcus erythropolis Mycobacterium avium

Protein Variants

Protein Variants Comment Organism
D123A no enzymic activity Mycobacterium avium
D123A mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
D250A no enzymic activity Mycobacterium avium
D250A mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
H279A no enzymic activity Mycobacterium avium
H279A mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
W124L no enzymic activity Mycobacterium avium
W124L mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
W164L no enzymic activity Mycobacterium avium
W164L mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 determined by SDS-PAGE Mycobacterium avium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-haloalkane + H2O Mycobacterium avium
-
 a primary alcohol + halide
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium avium
-
 expression in Rhodococcus erythropolis IAM 1399
-
Mycobacterium avium Q73Y99
-
-
Mycobacterium avium N85
-
 expression in Rhodococcus erythropolis IAM 1399
-

Purification (Commentary)

Purification (Comment) Organism
the cell free extract of Rhodococcus erythropolis IAM1399 cells is purified on a BD TALON metal affinity resin Mycobacterium avium

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism Mycobacterium avium
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00066
-
 1,2-dichloroethane Mycobacterium avium
0.6
-
 1-bromobutane Mycobacterium avium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dichloroethane + H2O
-
 Mycobacterium avium ?
-
 ?
1-bromobutane + H2O
-
 Mycobacterium avium n-butanol + bromide
-
 ?
1-bromobutane + H2O
-
 Mycobacterium avium 1-butanol + bromide
-
 ?
1-bromobutane + H2O
-
 Mycobacterium avium N85 1-butanol + bromide
-
 ?
1-haloalkane + H2O
-
 Mycobacterium avium a primary alcohol + halide
-
 ?

Synonyms

Synonyms Comment Organism
DhmA
-
 Mycobacterium avium
haloalkane dehalogenase
-
 Mycobacterium avium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 activity assay with 1,2-dichloroethane Mycobacterium avium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 activity assay with 1,2-dichloroethane Mycobacterium avium
8.6
-
 activity assay with 1-bromobutane Mycobacterium avium