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Literature summary for 3.8.1.5 extracted from

  • Sharma, P.; Raina, V.; Kumari, R.; Malhotra, S.; Dogra, C.; Kumari, H.; Kohler, H.P.; Buser, H.R.; Holliger, C.; Lal, R.
    Haloalkane dehalogenase LinB is responsible for beta- and delta-hexachlorocyclohexane transformation in Sphingobium indicum B90A (2006), Appl. Environ. Microbiol., 72, 5720-5727.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
into pGEX-5X-3 for expression in Escherichia coli BL21 cells Sphingobium indicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 GST fusion protein, determined by SDS-PAGE Sphingobium indicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-hexachlorocyclohexane + H2O Sphingobium indicum
-
 pentachlorocyclohexanol + chloride
-
 ?
delta-hexachlorocyclohexane + H2O Sphingobium indicum
-
 pentachlorocyclohexanol + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium indicum
-
-
-
Sphingobium indicum
-
 Sphingobium francense Sp+
-
Sphingobium indicum
-
 Sphingobium indicum B90A
-
Sphingobium indicum A8CFB7 Sphingobium japonicum UT26
-
Sphingobium indicum B90A
-
-
-
Sphingobium indicum Sp+
-
-
-
Sphingobium indicum UT26
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a glutathione S-transferase (GST)-glutathione affinity column chromatography kit, to remove the GST tag the protein is incubated with factor Xa Sphingobium indicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,4,5,6-pentachlorohexanol + H2O
-
 Sphingobium indicum tetrachlorocyclohexanediol + chloride
-
 ?
2,3,4,5,6-pentachlorohexanol + H2O
-
 Sphingobium indicum B90A tetrachlorocyclohexanediol + chloride
-
 ?
2,3,4,5,6-pentachlorohexanol + H2O
-
 Sphingobium indicum UT26 tetrachlorocyclohexanediol + chloride
-
 ?
2,3,4,5,6-pentachlorohexanol + H2O
-
 Sphingobium indicum Sp+ tetrachlorocyclohexanediol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O no further degradation of product Sphingobium indicum 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum B90A 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O no further degradation of product Sphingobium indicum B90A 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum UT26 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O no further degradation of product Sphingobium indicum UT26 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum Sp+ 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O no further degradation of product Sphingobium indicum Sp+ 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
beta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum pentachlorocyclohexanol + chloride
-
 ?
delta-hexachlorocyclohexane + H2O
-
 Sphingobium indicum pentachlorocyclohexanol + chloride
-
 ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum 2,3,4,5,6-pentachlorocyclohexanol + chloride no further degradation of product ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum B90A 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum B90A 2,3,4,5,6-pentachlorocyclohexanol + chloride no further degradation of product ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum UT26 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum UT26 2,3,4,5,6-pentachlorocyclohexanol + chloride no further degradation of product ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum Sp+ 2,3,4,5,6-pentachlorocyclohexanol + chloride
-
 ?
gamma-hexachlorocyclohexane + H2O
-
 Sphingobium indicum Sp+ 2,3,4,5,6-pentachlorocyclohexanol + chloride no further degradation of product ?
pentachlorocyclohexanol + H2O
-
 Sphingobium indicum tetrachlorocyclohexanediol + chloride
-
 ?

Synonyms

Synonyms Comment Organism
haloalkane dehalogenase
-
 Sphingobium indicum
LinB
-
 Sphingobium indicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Sphingobium indicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Sphingobium indicum