Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.8.1.5 extracted from

  • Jesenska, A.; Pavlova, M.; Strouhal, M.; Chaloupkova, R.; Tesinska, I.; Monincova, M.; Prokop, Z.; Bartos, M.; Pavlik, I.; Rychlik, I.; Moebius, P.; Nagata, Y.; Damborsky, J.
    Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases (2005), Appl. Environ. Microbiol., 71, 6736-6745.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis variant bovis

Inhibitors

Inhibitors Comment Organism Structure
potassium phosphate buffer
-
 Mycobacterium tuberculosis variant bovis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 1-Chlorobutane pH 8.5 Mycobacterium tuberculosis variant bovis
4.52
-
 1,3-dibromopropane pH 8.5 Mycobacterium tuberculosis variant bovis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
 x * 34500, SDS-PAGE Mycobacterium tuberculosis variant bovis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis variant bovis Q9XB14 isoform dmbA
-
Mycobacterium tuberculosis variant bovis 5033/66 Q9XB14 isoform dmbA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Mycobacterium tuberculosis variant bovis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dibromoethane + H2O
-
 Mycobacterium tuberculosis variant bovis 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Mycobacterium tuberculosis variant bovis 5033/66 2-bromoethanol + bromide
-
 ?
1,3-dibromopropane + H2O
-
 Mycobacterium tuberculosis variant bovis 3-bromo-propanol + bromide
-
 ?
1,3-dibromopropane + H2O
-
 Mycobacterium tuberculosis variant bovis 5033/66 3-bromo-propanol + bromide
-
 ?
1-chlorobutane + H2O
-
 Mycobacterium tuberculosis variant bovis butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Mycobacterium tuberculosis variant bovis 5033/66 butanol + chloride
-
 ?

Subunits

Subunits Comment Organism
? x * 34500, SDS-PAGE Mycobacterium tuberculosis variant bovis

Synonyms

Synonyms Comment Organism
dmbA
-
 Mycobacterium tuberculosis variant bovis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 32.5% of maximum activity Mycobacterium tuberculosis variant bovis
45
-
-
 Mycobacterium tuberculosis variant bovis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
 melting temperature Mycobacterium tuberculosis variant bovis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 1-Chlorobutane pH 8.5 Mycobacterium tuberculosis variant bovis
9.2
-
 1,3-dibromopropane pH 8.5 Mycobacterium tuberculosis variant bovis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 and also at pH 9.0, potassium phosphate buffer is inhibitory Mycobacterium tuberculosis variant bovis
9
-
 and also at pH 5.5, potassium phosphate buffer is inhibitory Mycobacterium tuberculosis variant bovis