Cloned (Comment) | Organism |
---|---|
- |
Bradyrhizobium japonicum |
Protein Variants | Comment | Organism |
---|---|---|
D103A | no activity with substrates 1,3-dibromopropane or 1-chlorobutane | Bradyrhizobium japonicum |
E127A | no activity with substrates 1,3-dibromopropane or 1-chlorobutane, purification of enzyme not possible because of very small amount of soluble enzyme protein | Bradyrhizobium japonicum |
H280A | no activity with substrates 1,3-dibromopropane or 1-chlorobutane | Bradyrhizobium japonicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-dibromopropane | substrate inhibition | Bradyrhizobium japonicum | |
3-chloro-2-methylpropene | substrate inhibition | Bradyrhizobium japonicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
chlorocyclohexane | - |
Bradyrhizobium japonicum | |
0.05 | - |
1-Chlorohexane | - |
Bradyrhizobium japonicum | |
0.11 | - |
bromocyclohexane | - |
Bradyrhizobium japonicum | |
0.22 | - |
1,3-dibromopropane | - |
Bradyrhizobium japonicum | |
0.28 | - |
1,2,3-trichloropropane | - |
Bradyrhizobium japonicum | |
0.47 | - |
3-chloro-2-methylpropene | - |
Bradyrhizobium japonicum | |
1.8 | - |
1,2-dibromopropane | - |
Bradyrhizobium japonicum | |
4 | - |
1-Chlorobutane | - |
Bradyrhizobium japonicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
strain USDA110 | - |
Bradyrhizobium japonicum USDA 110 | - |
strain USDA110 | - |
Mesorhizobium loti | - |
- |
- |
Mesorhizobium loti MAFF303099 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,3-trichloropropane + H2O | - |
Bradyrhizobium japonicum | ? | - |
? | |
1,2-dibromoethane + H2O | 1100% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | 2-bromoethanol + bromide | - |
? | |
1,2-dibromoethane + H2O | 1100% of the activity with 1-chlorobutane | Bradyrhizobium japonicum USDA 110 | 2-bromoethanol + bromide | - |
? | |
1,2-dibromopropane + H2O | - |
Bradyrhizobium japonicum | ? + bromide | - |
? | |
1,3-dibromopropane + H2O | 420% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | 3-bromopropanol + bromide | - |
? | |
1,3-dibromopropane + H2O | 700% of the activity with 1-chlorobutane | Mesorhizobium loti | 3-bromopropanol + bromide | - |
? | |
1-bromobutane + H2O | 180% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | n-butanol + bromide | - |
? | |
1-bromobutane + H2O | 630% of the activity with 1-chlorobutane | Mesorhizobium loti | n-butanol + bromide | - |
? | |
1-bromobutane + H2O | 180% of the activity with 1-chlorobutane | Bradyrhizobium japonicum USDA 110 | n-butanol + bromide | - |
? | |
1-bromobutane + H2O | 630% of the activity with 1-chlorobutane | Mesorhizobium loti MAFF303099 | n-butanol + bromide | - |
? | |
1-chlorobutane + H2O | - |
Bradyrhizobium japonicum | n-butanol + chloride | - |
? | |
1-chlorobutane + H2O | - |
Mesorhizobium loti | n-butanol + chloride | - |
? | |
1-chlorobutane + H2O | - |
Bradyrhizobium japonicum USDA 110 | n-butanol + chloride | - |
? | |
1-chlorobutane + H2O | - |
Mesorhizobium loti MAFF303099 | n-butanol + chloride | - |
? | |
1-chlorodecane + H2O | 110% of the activity with 1-chlorobutane | Mesorhizobium loti | n-decanol + chloride | - |
? | |
1-chlorodecane + H2O | 52% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | n-decanol + chloride | - |
? | |
1-chlorohexane + H2O | 110% of the activity with 1-chlorobutane | Mesorhizobium loti | n-hexanol + chloride | - |
? | |
1-chlorohexane + H2O | 210% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | n-hexanol + chloride | - |
? | |
1-chlorohexane + H2O | 210% of the activity with 1-chlorobutane | Bradyrhizobium japonicum USDA 110 | n-hexanol + chloride | - |
? | |
1-chlorohexane + H2O | 110% of the activity with 1-chlorobutane | Mesorhizobium loti MAFF303099 | n-hexanol + chloride | - |
? | |
1-chloropentane + H2O | 120% of the activity with 1-chlorobutane | Mesorhizobium loti | n-pentanol + chloride | - |
? | |
1-chloropentane + H2O | 140% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | n-pentanol + chloride | - |
? | |
3-chloro-2-methylpropene + H2O | 270% of the activity with 1-chlorobutane | Mesorhizobium loti | 2-methylpropene-3-ol + chloride | - |
? | |
3-chloro-2-methylpropene + H2O | 320% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | 2-methylpropene-3-ol + chloride | - |
? | |
3-chloro-2-methylpropene + H2O | 320% of the activity with 1-chlorobutane | Bradyrhizobium japonicum USDA 110 | 2-methylpropene-3-ol + chloride | - |
? | |
3-chloro-2-methylpropene + H2O | 270% of the activity with 1-chlorobutane | Mesorhizobium loti MAFF303099 | 2-methylpropene-3-ol + chloride | - |
? | |
bromocyclohexane + H2O | 420% of the activity with 1-chlorobutane | Mesorhizobium loti | cyclohexanol + bromide | - |
? | |
bromocyclohexane + H2O | 84% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | cyclohexanol + bromide | - |
? | |
bromocyclohexane + H2O | 420% of the activity with 1-chlorobutane | Mesorhizobium loti MAFF303099 | cyclohexanol + bromide | - |
? | |
chlorocyclohexane + H2O | 30% of the activity with 1-chlorobutane | Bradyrhizobium japonicum | cyclohexanol + chloride | - |
? | |
chlorocyclohexane + H2O | 620% of the activity with 1-chlorobutane | Mesorhizobium loti | cyclohexanol + chloride | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dmlA | - |
Bradyrhizobium japonicum |
dmlA | - |
Mesorhizobium loti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
1,2,3-trichloropropane | - |
Bradyrhizobium japonicum | |
0.25 | - |
chlorocyclohexane | - |
Bradyrhizobium japonicum | |
0.59 | - |
bromocyclohexane | - |
Bradyrhizobium japonicum | |
0.96 | - |
1,2-dibromopropane | - |
Bradyrhizobium japonicum | |
1.4 | - |
1-Chlorobutane | - |
Bradyrhizobium japonicum | |
1.4 | - |
1-Chlorohexane | - |
Bradyrhizobium japonicum | |
3.5 | - |
3-chloro-2-methylpropene | - |
Bradyrhizobium japonicum | |
3.6 | - |
1,3-dibromopropane | - |
Bradyrhizobium japonicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.12 | - |
3-chloro-2-methylpropene | - |
Bradyrhizobium japonicum | |
6.98 | - |
1,3-dibromopropane | - |
Bradyrhizobium japonicum |